Abstract
Thyroid hormone treatment in vivo results in activation of mitochondrial Ca2+ efflux and temperature-dependent enhanced swelling of Ca2+-loaded rat liver mitochondria. Thyroid hormone-induced swelling was effectively prevented in the presence of excess EGTA or cyclosporin A. Thyroid hormone treatment similarly resulted in a dramatic decrease in mitochondrial membrane potential (80%), proton gradient (45%), and proton motive force (69%) measured in Ca2+-loaded mitochondria. All three parameters were essentially restored to euthyroid values in the presence of excess EGTA or cyclosporin A. Mitochondrial energy-linked transhydrogenase activity measured in the presence of Ca2+ was 33% increased and 38% decreased in hypothyroid and L- T3-treated hypothyroid rats, respectively, compared to that in euthyroid rats. Hence, in vivo thyroid hormone treatment may induce mitochondrial permeability transition mediated by the cyclosporin A-sensitive permeability transition pore.
Original language | English |
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Pages (from-to) | 3552-3556 |
Number of pages | 5 |
Journal | Endocrinology |
Volume | 136 |
Issue number | 8 |
DOIs | |
State | Published - Aug 1995 |