TY - JOUR
T1 - Modification of cellular fatty acid composition of Hep-G2 cells
T2 - effect of antioxidants on cholesterol esterification and secretion
AU - Stein, O.
AU - Haratz, D.
AU - Shwartz, R.
AU - Berry, E. M.
AU - Stein, Y.
PY - 1989/6/8
Y1 - 1989/6/8
N2 - Modification of fatty acid composition of Hep-G2 cells was achieved by 7-9 days of supplementation of culture medium with palmitic, oleic or linoleic acid. Cholesterol release into serum-free culture medium during 24 h of incubation was significantly lower in cells supplemented with linoleic acid, when compared to those supplemented with palmitic, oleic or no additional fatty acid. In cells cultured in the presence of linoleic acid, less [3H]cholesterol was esterified to cholesteryl ester and the mass of cholesteryl ester was significantly lower than in cells cultured with palmitic acid or with no additional fatty acid. The reduction in [3H]cholesterol secretion and the impairment in cholesterol esterification in linoleic acid-treated cells was prevented by addition of butylated hydroxytoluene or probucol concurrently with the fatty acid. The antioxidants also increased esterification and [3H]cholesterol release in cells supplemented with the other fatty acids. It is suggested that cholesterol secretion and esterification are sensitive to peroxidation.
AB - Modification of fatty acid composition of Hep-G2 cells was achieved by 7-9 days of supplementation of culture medium with palmitic, oleic or linoleic acid. Cholesterol release into serum-free culture medium during 24 h of incubation was significantly lower in cells supplemented with linoleic acid, when compared to those supplemented with palmitic, oleic or no additional fatty acid. In cells cultured in the presence of linoleic acid, less [3H]cholesterol was esterified to cholesteryl ester and the mass of cholesteryl ester was significantly lower than in cells cultured with palmitic acid or with no additional fatty acid. The reduction in [3H]cholesterol secretion and the impairment in cholesterol esterification in linoleic acid-treated cells was prevented by addition of butylated hydroxytoluene or probucol concurrently with the fatty acid. The antioxidants also increased esterification and [3H]cholesterol release in cells supplemented with the other fatty acids. It is suggested that cholesterol secretion and esterification are sensitive to peroxidation.
KW - Butylated hydroxytoluene
KW - Cholesterol esterification
KW - Cholesterol secretion
KW - Linoleic acid
KW - Peroxidation
KW - Probucol
UR - http://www.scopus.com/inward/record.url?scp=0024307661&partnerID=8YFLogxK
U2 - 10.1016/0005-2760(89)90243-9
DO - 10.1016/0005-2760(89)90243-9
M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???
C2 - 2730885
AN - SCOPUS:0024307661
SN - 0005-2760
VL - 1003
SP - 115
EP - 120
JO - Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids
JF - Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids
IS - 2
ER -