Modified aminoacyl-tRNA. II. A general method for the preparation of acylaminoacyl-tRNA

Y. Lapidot; N. de Groot; I. Fry-Shafrir

doi:10.1016/0005-2787(67)90047-0

Modified aminoacyl-tRNA. II. A general method for the preparation of acylaminoacyl-tRNA

Y. Lapidot^*, N. de Groot, I. Fry-Shafrir

^*Corresponding author for this work

Alexander Silberman Institute of Life Sciences

Research output: Contribution to journal › Article › peer-review

48 Scopus citations

Abstract

A general and relatively simple method for the acylation of aminoacyl-tRNA is described. The synthesis involves a reaction between N-hydroxysuccinimide esters of short- and long-chain fatty acids and aminoacyl-tRNA. The activated fatty acids react specifically with the amino acid attached to the tRNA and do not react with the tRNA proper. The acylated phenylalanyl-tRNA did not lose its biological properties as a result of the acylation reaction. Acylphenylalanyl-tRNA could be bound to ribosomes in a poly (U)-dependent reaction. The tRNA could be recharged with phenylalanine after removal of the acylphenylalanine from the tRNA by mild alkaline hydrolysis.

Original language	English
Pages (from-to)	292-299
Number of pages	8
Journal	BBA Section Nucleic Acids And Protein Synthesis
Volume	145
Issue number	2
DOIs	https://doi.org/10.1016/0005-2787(67)90047-0
State	Published - 26 Sep 1967

Access to Document

10.1016/0005-2787(67)90047-0

Cite this

@article{198473e94961420aaa8e38a8a7060383,

title = "Modified aminoacyl-tRNA. II. A general method for the preparation of acylaminoacyl-tRNA",

abstract = "A general and relatively simple method for the acylation of aminoacyl-tRNA is described. The synthesis involves a reaction between N-hydroxysuccinimide esters of short- and long-chain fatty acids and aminoacyl-tRNA. The activated fatty acids react specifically with the amino acid attached to the tRNA and do not react with the tRNA proper. The acylated phenylalanyl-tRNA did not lose its biological properties as a result of the acylation reaction. Acylphenylalanyl-tRNA could be bound to ribosomes in a poly (U)-dependent reaction. The tRNA could be recharged with phenylalanine after removal of the acylphenylalanine from the tRNA by mild alkaline hydrolysis.",

author = "Y. Lapidot and {de Groot}, N. and I. Fry-Shafrir",

year = "1967",

month = sep,

day = "26",

doi = "10.1016/0005-2787(67)90047-0",

language = "אנגלית",

volume = "145",

pages = "292--299",

journal = "BBA Section Nucleic Acids And Protein Synthesis",

issn = "0005-2787",

publisher = "Elsevier B.V.",

number = "2",

}

TY - JOUR

T1 - Modified aminoacyl-tRNA. II. A general method for the preparation of acylaminoacyl-tRNA

AU - Lapidot, Y.

AU - de Groot, N.

AU - Fry-Shafrir, I.

PY - 1967/9/26

Y1 - 1967/9/26

N2 - A general and relatively simple method for the acylation of aminoacyl-tRNA is described. The synthesis involves a reaction between N-hydroxysuccinimide esters of short- and long-chain fatty acids and aminoacyl-tRNA. The activated fatty acids react specifically with the amino acid attached to the tRNA and do not react with the tRNA proper. The acylated phenylalanyl-tRNA did not lose its biological properties as a result of the acylation reaction. Acylphenylalanyl-tRNA could be bound to ribosomes in a poly (U)-dependent reaction. The tRNA could be recharged with phenylalanine after removal of the acylphenylalanine from the tRNA by mild alkaline hydrolysis.

AB - A general and relatively simple method for the acylation of aminoacyl-tRNA is described. The synthesis involves a reaction between N-hydroxysuccinimide esters of short- and long-chain fatty acids and aminoacyl-tRNA. The activated fatty acids react specifically with the amino acid attached to the tRNA and do not react with the tRNA proper. The acylated phenylalanyl-tRNA did not lose its biological properties as a result of the acylation reaction. Acylphenylalanyl-tRNA could be bound to ribosomes in a poly (U)-dependent reaction. The tRNA could be recharged with phenylalanine after removal of the acylphenylalanine from the tRNA by mild alkaline hydrolysis.

UR - http://www.scopus.com/inward/record.url?scp=0014218032&partnerID=8YFLogxK

U2 - 10.1016/0005-2787(67)90047-0

DO - 10.1016/0005-2787(67)90047-0

M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???

C2 - 6064630

AN - SCOPUS:0014218032

SN - 0005-2787

VL - 145

SP - 292

EP - 299

JO - BBA Section Nucleic Acids And Protein Synthesis

JF - BBA Section Nucleic Acids And Protein Synthesis

IS - 2

ER -

Modified aminoacyl-tRNA. II. A general method for the preparation of acylaminoacyl-tRNA

Abstract

Access to Document

Other files and links

Fingerprint

Cite this