Modified aminoacyl-tRNA. II. A general method for the preparation of acylaminoacyl-tRNA

Y. Lapidot; N. de Groot; I. Fry-Shafrir

doi:10.1016/0005-2787(67)90047-0

Modified aminoacyl-tRNA. II. A general method for the preparation of acylaminoacyl-tRNA

Y. Lapidot^*
, N. de Groot
, I. Fry-Shafrir

^*Corresponding author for this work

Alexander Silberman Institute of Life Sciences

Research output: Contribution to journal › Article › peer-review

48 Scopus citations

Abstract

A general and relatively simple method for the acylation of aminoacyl-tRNA is described. The synthesis involves a reaction between N-hydroxysuccinimide esters of short- and long-chain fatty acids and aminoacyl-tRNA. The activated fatty acids react specifically with the amino acid attached to the tRNA and do not react with the tRNA proper. The acylated phenylalanyl-tRNA did not lose its biological properties as a result of the acylation reaction. Acylphenylalanyl-tRNA could be bound to ribosomes in a poly (U)-dependent reaction. The tRNA could be recharged with phenylalanine after removal of the acylphenylalanine from the tRNA by mild alkaline hydrolysis.

Original language	English
Pages (from-to)	292-299
Number of pages	8
Journal	BBA Section Nucleic Acids And Protein Synthesis
Volume	145
Issue number	2
DOIs	https://doi.org/10.1016/0005-2787(67)90047-0
State	Published - 26 Sep 1967

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abstract = "A general and relatively simple method for the acylation of aminoacyl-tRNA is described. The synthesis involves a reaction between N-hydroxysuccinimide esters of short- and long-chain fatty acids and aminoacyl-tRNA. The activated fatty acids react specifically with the amino acid attached to the tRNA and do not react with the tRNA proper. The acylated phenylalanyl-tRNA did not lose its biological properties as a result of the acylation reaction. Acylphenylalanyl-tRNA could be bound to ribosomes in a poly (U)-dependent reaction. The tRNA could be recharged with phenylalanine after removal of the acylphenylalanine from the tRNA by mild alkaline hydrolysis.",

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Modified aminoacyl-tRNA. II. A general method for the preparation of acylaminoacyl-tRNA

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