TY - JOUR
T1 - Modified aminoacyl-tRNA. IV. The behaviour of acyl[14C]phenylalanyl-tRNA in a ribosomal system from wheat germ
AU - De Groot, N.
AU - Panet, A.
AU - Fry-Shafrir, I.
AU - Lapidot, Y.
PY - 1968/6/18
Y1 - 1968/6/18
N2 - The binding of acyl[14C]phenylalanyl-tRNA to wheat germ ribosomes was studied. Acyl[14C]phenylalanyl-tRNA binds less tightly to the ribosomes in the presence of poly U than the parent [14C]phenylalanyl-tRNA. Uncharged tRNA inhibits the binding of acyl[14C]phenylalanyl-tRNA more than the binding of [14C]phenylalanyl-tRNA. GTP, at a low concentration (10 μM), inhibits the binding of acyl[14C]phenylalanyl-tRNA but not that of [14C]phenylalanyl-tRNA. A 14C-labelled peptide is formed from acyl[14C]phenylalanyl-tRNA only if ribosomes, poly U and acyl[14C]phenylalanyl-tRNA are incubated prior to adding all the other components of the incorporation reaction. The peptide formed from acyl[14C]phenylalanyl-tRNA has a blocked terminal amino group and internal phenylalanyl residues are labelled. At 0.004 M magnesium acetate, the amount of [14C]phenylalanine incorporated from acyl[14C]phenylalanyl-tRNA is higher than that from [14C]phenylalanyl-tRNA under identical reaction conditions.
AB - The binding of acyl[14C]phenylalanyl-tRNA to wheat germ ribosomes was studied. Acyl[14C]phenylalanyl-tRNA binds less tightly to the ribosomes in the presence of poly U than the parent [14C]phenylalanyl-tRNA. Uncharged tRNA inhibits the binding of acyl[14C]phenylalanyl-tRNA more than the binding of [14C]phenylalanyl-tRNA. GTP, at a low concentration (10 μM), inhibits the binding of acyl[14C]phenylalanyl-tRNA but not that of [14C]phenylalanyl-tRNA. A 14C-labelled peptide is formed from acyl[14C]phenylalanyl-tRNA only if ribosomes, poly U and acyl[14C]phenylalanyl-tRNA are incubated prior to adding all the other components of the incorporation reaction. The peptide formed from acyl[14C]phenylalanyl-tRNA has a blocked terminal amino group and internal phenylalanyl residues are labelled. At 0.004 M magnesium acetate, the amount of [14C]phenylalanine incorporated from acyl[14C]phenylalanyl-tRNA is higher than that from [14C]phenylalanyl-tRNA under identical reaction conditions.
UR - http://www.scopus.com/inward/record.url?scp=0014422191&partnerID=8YFLogxK
U2 - 10.1016/0005-2787(68)90302-X
DO - 10.1016/0005-2787(68)90302-X
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C2 - 5661364
AN - SCOPUS:0014422191
SN - 0005-2787
VL - 161
SP - 137
EP - 146
JO - BBA Section Nucleic Acids And Protein Synthesis
JF - BBA Section Nucleic Acids And Protein Synthesis
IS - 1
ER -