TY - JOUR
T1 - Modulation of the expression of CD4 on HL-60 cells by exposure to 1,25-dihydroxyvitamin D3
AU - Schlesinger, Michael
AU - Bar-Shavit, Zvi
AU - Hadar, Rivka
AU - Rabinowitz, Ruth
PY - 1989/10
Y1 - 1989/10
N2 - The CD4 molecule functions as a receptor for the binding and infectivity of the human immunodeficiency virus (HIV). It is of interest, therefore, to develop procedures for its down-regulation. In the present study, the effect of 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) on the expression of cell surface antigens of the HL-60 promyelocytic leukemia cell line was analyzed. Exposure of HL-60 cells to 1,25(OH)2D3 resulted in down-regulation of CD4 as assessed by their staining with the Leu-3a monoclonal antibody (MoAb). This treatment increased the staining of HL-60 cells with the monocyte-specific 63D3 MoAb. In contrast to the rapid elimination of cell surface CD4 by exposure of HL-60 to phorbol myristate acetate (PMA), the maximal reduction of CD4 by 1,25(OH)2D3 was attained within 48 h after the beginning of the exposure.
AB - The CD4 molecule functions as a receptor for the binding and infectivity of the human immunodeficiency virus (HIV). It is of interest, therefore, to develop procedures for its down-regulation. In the present study, the effect of 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) on the expression of cell surface antigens of the HL-60 promyelocytic leukemia cell line was analyzed. Exposure of HL-60 cells to 1,25(OH)2D3 resulted in down-regulation of CD4 as assessed by their staining with the Leu-3a monoclonal antibody (MoAb). This treatment increased the staining of HL-60 cells with the monocyte-specific 63D3 MoAb. In contrast to the rapid elimination of cell surface CD4 by exposure of HL-60 to phorbol myristate acetate (PMA), the maximal reduction of CD4 by 1,25(OH)2D3 was attained within 48 h after the beginning of the exposure.
KW - 1,25-Dihydroxyvitamin D
KW - CD4
KW - Modulation
KW - Monoclonal antibody
UR - http://www.scopus.com/inward/record.url?scp=0024470723&partnerID=8YFLogxK
U2 - 10.1016/0165-2478(89)90170-3
DO - 10.1016/0165-2478(89)90170-3
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C2 - 2697687
AN - SCOPUS:0024470723
SN - 0165-2478
VL - 22
SP - 307
EP - 311
JO - Immunology Letters
JF - Immunology Letters
IS - 4
ER -