Modulation of the expression of CD4 on HL-60 cells by exposure to 1,25-dihydroxyvitamin D3

Michael Schlesinger*, Zvi Bar-Shavit, Rivka Hadar, Ruth Rabinowitz

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

The CD4 molecule functions as a receptor for the binding and infectivity of the human immunodeficiency virus (HIV). It is of interest, therefore, to develop procedures for its down-regulation. In the present study, the effect of 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) on the expression of cell surface antigens of the HL-60 promyelocytic leukemia cell line was analyzed. Exposure of HL-60 cells to 1,25(OH)2D3 resulted in down-regulation of CD4 as assessed by their staining with the Leu-3a monoclonal antibody (MoAb). This treatment increased the staining of HL-60 cells with the monocyte-specific 63D3 MoAb. In contrast to the rapid elimination of cell surface CD4 by exposure of HL-60 to phorbol myristate acetate (PMA), the maximal reduction of CD4 by 1,25(OH)2D3 was attained within 48 h after the beginning of the exposure.

Original languageEnglish
Pages (from-to)307-311
Number of pages5
JournalImmunology Letters
Volume22
Issue number4
DOIs
StatePublished - Oct 1989

Keywords

  • 1,25-Dihydroxyvitamin D
  • CD4
  • Modulation
  • Monoclonal antibody

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