TY - JOUR
T1 - Molecular characterisation of Onion yellow dwarf virus (OYDV) infecting garlic (Allium sativum L.) in Israel
T2 - Thermotherapy inhibits virus elimination by meristem tip culture
AU - Shiboleth, Y. M.
AU - Gal-On, A.
AU - Koch, M.
AU - Rabinowitch, H. D.
AU - Salomon, R.
PY - 2001
Y1 - 2001
N2 - Garlic (cv. Shani) was tested using single step RT-PCR and digoxygenin (DIG) labelled dot-blot for a number of viruses. Following sequence analysis it was shown that at least three different polymorphs of the potyvirus Onion yellow dwarf virus (OYDV) infect the same plant simultaneously, together with the potyvirus Leek yellow stripe virus (LYSV), the carlavirus Garlic common latent virus (GCLV) and a multitude of allexiviruses [Shallot virus X (ShVX) related viruses]. Several garlic plants free of all the viruses tested were obtained through meristem-tip culture. Plants infected with single viruses or with different combinations of viruses were similarly obtained. Meristem-tip culture was confirmed as a satisfactory method of virus eradication, while thermotherapy treatment given to mother plantlets before meristem excision was found to specifically antagonise OYDV eradication. This work uses molecular methods for the first time to examine the effectiveness of meristem-tip culture for the eradication of multiple viruses from garlic.
AB - Garlic (cv. Shani) was tested using single step RT-PCR and digoxygenin (DIG) labelled dot-blot for a number of viruses. Following sequence analysis it was shown that at least three different polymorphs of the potyvirus Onion yellow dwarf virus (OYDV) infect the same plant simultaneously, together with the potyvirus Leek yellow stripe virus (LYSV), the carlavirus Garlic common latent virus (GCLV) and a multitude of allexiviruses [Shallot virus X (ShVX) related viruses]. Several garlic plants free of all the viruses tested were obtained through meristem-tip culture. Plants infected with single viruses or with different combinations of viruses were similarly obtained. Meristem-tip culture was confirmed as a satisfactory method of virus eradication, while thermotherapy treatment given to mother plantlets before meristem excision was found to specifically antagonise OYDV eradication. This work uses molecular methods for the first time to examine the effectiveness of meristem-tip culture for the eradication of multiple viruses from garlic.
KW - Allexivirus
KW - Allium sativum
KW - Garlic
KW - Garlic common latent virus (GCLV)
KW - Leek yellow stripe virus (LYSV)
KW - Meristem tip culture
KW - Onion yellow dwarf virus (OYDV)
KW - Reverse transcriptase polymerase chain reaction (RT-PCR)
KW - Shallot virus X (ShVX)
KW - Thermotherapy
KW - Virus eradication
KW - Virus-free
UR - http://www.scopus.com/inward/record.url?scp=0034946591&partnerID=8YFLogxK
U2 - 10.1111/j.1744-7348.2001.tb00101.x
DO - 10.1111/j.1744-7348.2001.tb00101.x
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AN - SCOPUS:0034946591
SN - 0003-4746
VL - 138
SP - 187
EP - 195
JO - Annals of Applied Biology
JF - Annals of Applied Biology
IS - 2
ER -