Molecular characterization, expression and immune functions of two C-type lectin from Venerupis philippinarum

Zifan Xin; Daode Yu; Bowen Yang; Lizhu Chen; Zvi Hayouka; Xingfu Chen; Yitong Gong; Haiyu Dai; Lei Wang; Yancui Zhao; Xiaoli Liu; Dinglong Yang

doi:10.1016/j.fsi.2020.10.006

Molecular characterization, expression and immune functions of two C-type lectin from Venerupis philippinarum

Zifan Xin, Daode Yu, Bowen Yang, Lizhu Chen, Zvi Hayouka, Xingfu Chen, Yitong Gong, Haiyu Dai, Lei Wang, Yancui Zhao, Xiaoli Liu^*, Dinglong Yang^*

^*Corresponding author for this work

Institute of Biochemistry, Food Science and Nutrition

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

In the present study, two C-type lectins (designated as VpClec-3 and VpClec-4) were identified and characterized from the manila clam Venerupis philippinarum. Multiple alignment and phylogenetic relationship analysis strongly suggested that VpClec-3 and VpClec-4 belong to the C-type lectin family. In nonstimulated clams, the VpClec-3 transcript was dominantly expressed in the hepatopancreas, while the VpClec-4 transcript was mainly expressed in gill tissues. Both VpClec-3 and VpClec-4 mRNA expression was significantly upregulated following Vibrio anguillarum challenge. Recombinant VpClec-4 (rVpClec-4) was shown to bind lipopolysaccharide (LPS) and glucan in vitro, whereas recombinant VpClec-3 (rVpClec-3) only bound to glucan. In addition, rVpClec-3 and rVpClec-4 displayed broad agglutination activities towards Vibrio harveyi, Vibrio splendidus and V. anguillarum, while no agglutination activities towards Enterobacter cloacae or Aeromonas hydrophila were observed in rVpClec-3. Moreover, hemocyte phagocytosis was significantly enhanced by rVpClec-3 and rVpClec-4. All the results showed that VpClecs function as pattern recognition receptors (PRRs) with distinct recognition spectra and are potentially involved in the innate immune responses of V. philippinarum.

Original language	American English
Pages (from-to)	260-268
Number of pages	9
Journal	Fish and Shellfish Immunology
Volume	107
DOIs	https://doi.org/10.1016/j.fsi.2020.10.006
State	Published - Dec 2020

Bibliographical note

Funding Information:
This research was supported by grants from the Strategic Priority Research Program of the Chinese Academy of Sciences ( XDA23050303 ), the National Natural Science Foundation of China (No. 41806196 ; 41506190 ), Natural Science Foundation of Shandong Province ( ZR2019BD022 , ZR2017MC045 ), Yantai Science and Technology Development Project ( 2020MSGY066 ) and Marine and Fisheries Science and Technology Innovation Program of Shandong ( 2017YY04 ).

Publisher Copyright:
© 2020 Elsevier Ltd

Keywords

C-type lectin
Immune recognition
Molecular characterization
Venerupis philippinarum

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@article{8088b7f6f9de425388f4fab6cace83f0,

title = "Molecular characterization, expression and immune functions of two C-type lectin from Venerupis philippinarum",

abstract = "In the present study, two C-type lectins (designated as VpClec-3 and VpClec-4) were identified and characterized from the manila clam Venerupis philippinarum. Multiple alignment and phylogenetic relationship analysis strongly suggested that VpClec-3 and VpClec-4 belong to the C-type lectin family. In nonstimulated clams, the VpClec-3 transcript was dominantly expressed in the hepatopancreas, while the VpClec-4 transcript was mainly expressed in gill tissues. Both VpClec-3 and VpClec-4 mRNA expression was significantly upregulated following Vibrio anguillarum challenge. Recombinant VpClec-4 (rVpClec-4) was shown to bind lipopolysaccharide (LPS) and glucan in vitro, whereas recombinant VpClec-3 (rVpClec-3) only bound to glucan. In addition, rVpClec-3 and rVpClec-4 displayed broad agglutination activities towards Vibrio harveyi, Vibrio splendidus and V. anguillarum, while no agglutination activities towards Enterobacter cloacae or Aeromonas hydrophila were observed in rVpClec-3. Moreover, hemocyte phagocytosis was significantly enhanced by rVpClec-3 and rVpClec-4. All the results showed that VpClecs function as pattern recognition receptors (PRRs) with distinct recognition spectra and are potentially involved in the innate immune responses of V. philippinarum.",

keywords = "C-type lectin, Immune recognition, Molecular characterization, Venerupis philippinarum",

author = "Zifan Xin and Daode Yu and Bowen Yang and Lizhu Chen and Zvi Hayouka and Xingfu Chen and Yitong Gong and Haiyu Dai and Lei Wang and Yancui Zhao and Xiaoli Liu and Dinglong Yang",

note = "Funding Information: This research was supported by grants from the Strategic Priority Research Program of the Chinese Academy of Sciences ( XDA23050303 ), the National Natural Science Foundation of China (No. 41806196 ; 41506190 ), Natural Science Foundation of Shandong Province ( ZR2019BD022 , ZR2017MC045 ), Yantai Science and Technology Development Project ( 2020MSGY066 ) and Marine and Fisheries Science and Technology Innovation Program of Shandong ( 2017YY04 ). Publisher Copyright: {\textcopyright} 2020 Elsevier Ltd",

year = "2020",

month = dec,

doi = "10.1016/j.fsi.2020.10.006",

language = "American English",

volume = "107",

pages = "260--268",

journal = "Fish and Shellfish Immunology",

issn = "1050-4648",

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TY - JOUR

T1 - Molecular characterization, expression and immune functions of two C-type lectin from Venerupis philippinarum

AU - Xin, Zifan

AU - Yu, Daode

AU - Yang, Bowen

AU - Chen, Lizhu

AU - Hayouka, Zvi

AU - Chen, Xingfu

AU - Gong, Yitong

AU - Dai, Haiyu

AU - Wang, Lei

AU - Zhao, Yancui

AU - Liu, Xiaoli

AU - Yang, Dinglong

N1 - Funding Information: This research was supported by grants from the Strategic Priority Research Program of the Chinese Academy of Sciences ( XDA23050303 ), the National Natural Science Foundation of China (No. 41806196 ; 41506190 ), Natural Science Foundation of Shandong Province ( ZR2019BD022 , ZR2017MC045 ), Yantai Science and Technology Development Project ( 2020MSGY066 ) and Marine and Fisheries Science and Technology Innovation Program of Shandong ( 2017YY04 ). Publisher Copyright: © 2020 Elsevier Ltd

PY - 2020/12

Y1 - 2020/12

N2 - In the present study, two C-type lectins (designated as VpClec-3 and VpClec-4) were identified and characterized from the manila clam Venerupis philippinarum. Multiple alignment and phylogenetic relationship analysis strongly suggested that VpClec-3 and VpClec-4 belong to the C-type lectin family. In nonstimulated clams, the VpClec-3 transcript was dominantly expressed in the hepatopancreas, while the VpClec-4 transcript was mainly expressed in gill tissues. Both VpClec-3 and VpClec-4 mRNA expression was significantly upregulated following Vibrio anguillarum challenge. Recombinant VpClec-4 (rVpClec-4) was shown to bind lipopolysaccharide (LPS) and glucan in vitro, whereas recombinant VpClec-3 (rVpClec-3) only bound to glucan. In addition, rVpClec-3 and rVpClec-4 displayed broad agglutination activities towards Vibrio harveyi, Vibrio splendidus and V. anguillarum, while no agglutination activities towards Enterobacter cloacae or Aeromonas hydrophila were observed in rVpClec-3. Moreover, hemocyte phagocytosis was significantly enhanced by rVpClec-3 and rVpClec-4. All the results showed that VpClecs function as pattern recognition receptors (PRRs) with distinct recognition spectra and are potentially involved in the innate immune responses of V. philippinarum.

AB - In the present study, two C-type lectins (designated as VpClec-3 and VpClec-4) were identified and characterized from the manila clam Venerupis philippinarum. Multiple alignment and phylogenetic relationship analysis strongly suggested that VpClec-3 and VpClec-4 belong to the C-type lectin family. In nonstimulated clams, the VpClec-3 transcript was dominantly expressed in the hepatopancreas, while the VpClec-4 transcript was mainly expressed in gill tissues. Both VpClec-3 and VpClec-4 mRNA expression was significantly upregulated following Vibrio anguillarum challenge. Recombinant VpClec-4 (rVpClec-4) was shown to bind lipopolysaccharide (LPS) and glucan in vitro, whereas recombinant VpClec-3 (rVpClec-3) only bound to glucan. In addition, rVpClec-3 and rVpClec-4 displayed broad agglutination activities towards Vibrio harveyi, Vibrio splendidus and V. anguillarum, while no agglutination activities towards Enterobacter cloacae or Aeromonas hydrophila were observed in rVpClec-3. Moreover, hemocyte phagocytosis was significantly enhanced by rVpClec-3 and rVpClec-4. All the results showed that VpClecs function as pattern recognition receptors (PRRs) with distinct recognition spectra and are potentially involved in the innate immune responses of V. philippinarum.

KW - C-type lectin

KW - Immune recognition

KW - Molecular characterization

KW - Venerupis philippinarum

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U2 - 10.1016/j.fsi.2020.10.006

DO - 10.1016/j.fsi.2020.10.006

M3 - Article

C2 - 33031900

AN - SCOPUS:85092801259

SN - 1050-4648

VL - 107

SP - 260

EP - 268

JO - Fish and Shellfish Immunology

JF - Fish and Shellfish Immunology

ER -

Molecular characterization, expression and immune functions of two C-type lectin from Venerupis philippinarum

Abstract

Bibliographical note

Keywords

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