Molecular characterization, expression and immune functions of two C-type lectin from Venerupis philippinarum

Zifan Xin, Daode Yu, Bowen Yang, Lizhu Chen, Zvi Hayouka, Xingfu Chen, Yitong Gong, Haiyu Dai, Lei Wang, Yancui Zhao, Xiaoli Liu*, Dinglong Yang*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

In the present study, two C-type lectins (designated as VpClec-3 and VpClec-4) were identified and characterized from the manila clam Venerupis philippinarum. Multiple alignment and phylogenetic relationship analysis strongly suggested that VpClec-3 and VpClec-4 belong to the C-type lectin family. In nonstimulated clams, the VpClec-3 transcript was dominantly expressed in the hepatopancreas, while the VpClec-4 transcript was mainly expressed in gill tissues. Both VpClec-3 and VpClec-4 mRNA expression was significantly upregulated following Vibrio anguillarum challenge. Recombinant VpClec-4 (rVpClec-4) was shown to bind lipopolysaccharide (LPS) and glucan in vitro, whereas recombinant VpClec-3 (rVpClec-3) only bound to glucan. In addition, rVpClec-3 and rVpClec-4 displayed broad agglutination activities towards Vibrio harveyi, Vibrio splendidus and V. anguillarum, while no agglutination activities towards Enterobacter cloacae or Aeromonas hydrophila were observed in rVpClec-3. Moreover, hemocyte phagocytosis was significantly enhanced by rVpClec-3 and rVpClec-4. All the results showed that VpClecs function as pattern recognition receptors (PRRs) with distinct recognition spectra and are potentially involved in the innate immune responses of V. philippinarum.

Original languageAmerican English
Pages (from-to)260-268
Number of pages9
JournalFish and Shellfish Immunology
Volume107
DOIs
StatePublished - Dec 2020

Bibliographical note

Publisher Copyright:
© 2020 Elsevier Ltd

Keywords

  • C-type lectin
  • Immune recognition
  • Molecular characterization
  • Venerupis philippinarum

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