Abstract
The pediocin A-encoding plasmid of Pediococcus pentosaceus 43200, pMD136, was characterized by restriction enzyme analysis. Analysis of its replicon was facilitated by the construction of a probe vector consisting of the Escherichia coli plasmid pSP72 and the cat gene from Staphylococcus aureus plasmid pC194. The replication region of pMD136 was localized on a 1.6-kb EcoRI/BglII fragment. Sequencing analysis revealed a non-coding region, repA, spanning the first 440 bp, followed by an open reading frame, repB, encoding a putative protein of 390 amino acids. The non-coding region contained two sets of 6-bp and two sets of 22-bp direct repeats and two sets of inverted repeals upstream of the open reading frame. Strong homology of the isolated replicon was found to theta-type replicons of Lactococcus lactis plasmids. Segregational stability assay suggested at least two regions as potentially involved in the stabilization of pMD136. The plasmid's strong homology to other theta-type replicons and its relatively high stability suggest that pMD136 belongs to the widespread family of theta-replication plasmids.
Original language | English |
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Pages (from-to) | 237-244 |
Number of pages | 8 |
Journal | FEMS Microbiology Letters |
Volume | 151 |
Issue number | 2 |
DOIs | |
State | Published - 15 Jun 1997 |
Bibliographical note
Funding Information:This work was supported by the U.S.-Israel Binational Agriculture Research and Development Fund (No. US-2113-92).
Keywords
- Pediococcus pentosaceus
- Repeated sequence
- Segregational stability
- Theta- type replicons