Molecular characterization of the replicon of the Pediococcus pentosaceus 43200 pediocin A plasmid pMD136

Ayelet Kantor, Thomas J. Montville, Anait Mett, Roni Shapira*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

16 Scopus citations


The pediocin A-encoding plasmid of Pediococcus pentosaceus 43200, pMD136, was characterized by restriction enzyme analysis. Analysis of its replicon was facilitated by the construction of a probe vector consisting of the Escherichia coli plasmid pSP72 and the cat gene from Staphylococcus aureus plasmid pC194. The replication region of pMD136 was localized on a 1.6-kb EcoRI/BglII fragment. Sequencing analysis revealed a non-coding region, repA, spanning the first 440 bp, followed by an open reading frame, repB, encoding a putative protein of 390 amino acids. The non-coding region contained two sets of 6-bp and two sets of 22-bp direct repeats and two sets of inverted repeals upstream of the open reading frame. Strong homology of the isolated replicon was found to theta-type replicons of Lactococcus lactis plasmids. Segregational stability assay suggested at least two regions as potentially involved in the stabilization of pMD136. The plasmid's strong homology to other theta-type replicons and its relatively high stability suggest that pMD136 belongs to the widespread family of theta-replication plasmids.

Original languageAmerican English
Pages (from-to)237-244
Number of pages8
JournalFEMS Microbiology Letters
Issue number2
StatePublished - 15 Jun 1997

Bibliographical note

Funding Information:
This work was supported by the U.S.-Israel Binational Agriculture Research and Development Fund (No. US-2113-92).


  • Pediococcus pentosaceus
  • Repeated sequence
  • Segregational stability
  • Theta- type replicons


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