Molecular cloning and characterization of five annexin genes from Indian mustard (Brassica juncea L. Czern and Coss)

Sravan Kumar Jami; Ahan Dalal; K. Divya; P. B. Kirti

doi:10.1016/j.plaphy.2009.08.005

Molecular cloning and characterization of five annexin genes from Indian mustard (Brassica juncea L. Czern and Coss)

Sravan Kumar Jami^*, Ahan Dalal, K. Divya, P. B. Kirti

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

43 Scopus citations

Abstract

Plant annexins constitute a multigene family having suggested roles in a variety of cellular processes including stress responses. We have isolated and characterized five different cDNAs of mustard, Brassica juncea (AnnBj1, AnnBj2, AnnBj3, AnnBj6 and AnnBj7) encoding annexin proteins using a RT-PCR/RACE-PCR based strategy. The predicted molecular masses of these annexins are ∼36.0 kDa with acidic pIs. At the amino acid level, they share high sequence similarity with each other and with annexins from higher plants. Phylogenetic analysis revealed their evolutionary relationship with corresponding orthologous sequences in Arabidopsis and deduced proteins in various plant species. Expression analysis by semi-quantitative RT-PCR revealed that these genes are differentially expressed in various tissues. The expression patterns of these genes also showed regulation by various stress conditions such as exposure to signaling molecules, salinity and oxidative stress and wounding. Additionally, the in silico promoter analysis (of AnnBj1, AnnBj2 and AnnBj3) showed the presence of different cis-responsive elements that could respond to various stress conditions. These results indicate that AnnBj genes may play important roles in adaptation of plants to various environmental stresses.

Original language	English
Pages (from-to)	977-990
Number of pages	14
Journal	Plant Physiology and Biochemistry
Volume	47
Issue number	11-12
DOIs	https://doi.org/10.1016/j.plaphy.2009.08.005
State	Published - Nov 2009
Externally published	Yes

Bibliographical note

Funding Information:
The authors thank for this research support by the AP-Netherlands Biotechnology program operated by the Biotechnology Unit, Institute of Public Enterprise, Hyderabad, India. SKJ and KD are thankful to Council of Scientific and Industrial Research (CSIR), Government of India for financial assistance in the form of a research fellowship. We thank Greg Clark and Doug Durnin for critically reading the manuscript. SKJ is grateful to Dr. Robert Hill, Department of Plant Science, University of Manitoba for the lab facilities for some of the experiments. The departmental facilities funded by UGC-SAP/CAS, COSIST and DST-FIST-II are gratefully acknowledged.

Keywords

Abiotic stress
Annexin
Brassica juncea
Calcium-binding protein

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10.1016/j.plaphy.2009.08.005

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title = "Molecular cloning and characterization of five annexin genes from Indian mustard (Brassica juncea L. Czern and Coss)",

abstract = "Plant annexins constitute a multigene family having suggested roles in a variety of cellular processes including stress responses. We have isolated and characterized five different cDNAs of mustard, Brassica juncea (AnnBj1, AnnBj2, AnnBj3, AnnBj6 and AnnBj7) encoding annexin proteins using a RT-PCR/RACE-PCR based strategy. The predicted molecular masses of these annexins are ∼36.0 kDa with acidic pIs. At the amino acid level, they share high sequence similarity with each other and with annexins from higher plants. Phylogenetic analysis revealed their evolutionary relationship with corresponding orthologous sequences in Arabidopsis and deduced proteins in various plant species. Expression analysis by semi-quantitative RT-PCR revealed that these genes are differentially expressed in various tissues. The expression patterns of these genes also showed regulation by various stress conditions such as exposure to signaling molecules, salinity and oxidative stress and wounding. Additionally, the in silico promoter analysis (of AnnBj1, AnnBj2 and AnnBj3) showed the presence of different cis-responsive elements that could respond to various stress conditions. These results indicate that AnnBj genes may play important roles in adaptation of plants to various environmental stresses.",

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author = "Jami, {Sravan Kumar} and Ahan Dalal and K. Divya and Kirti, {P. B.}",

note = "Funding Information: The authors thank for this research support by the AP-Netherlands Biotechnology program operated by the Biotechnology Unit, Institute of Public Enterprise, Hyderabad, India. SKJ and KD are thankful to Council of Scientific and Industrial Research (CSIR), Government of India for financial assistance in the form of a research fellowship. We thank Greg Clark and Doug Durnin for critically reading the manuscript. SKJ is grateful to Dr. Robert Hill, Department of Plant Science, University of Manitoba for the lab facilities for some of the experiments. The departmental facilities funded by UGC-SAP/CAS, COSIST and DST-FIST-II are gratefully acknowledged. ",

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AU - Kirti, P. B.

N1 - Funding Information: The authors thank for this research support by the AP-Netherlands Biotechnology program operated by the Biotechnology Unit, Institute of Public Enterprise, Hyderabad, India. SKJ and KD are thankful to Council of Scientific and Industrial Research (CSIR), Government of India for financial assistance in the form of a research fellowship. We thank Greg Clark and Doug Durnin for critically reading the manuscript. SKJ is grateful to Dr. Robert Hill, Department of Plant Science, University of Manitoba for the lab facilities for some of the experiments. The departmental facilities funded by UGC-SAP/CAS, COSIST and DST-FIST-II are gratefully acknowledged.

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N2 - Plant annexins constitute a multigene family having suggested roles in a variety of cellular processes including stress responses. We have isolated and characterized five different cDNAs of mustard, Brassica juncea (AnnBj1, AnnBj2, AnnBj3, AnnBj6 and AnnBj7) encoding annexin proteins using a RT-PCR/RACE-PCR based strategy. The predicted molecular masses of these annexins are ∼36.0 kDa with acidic pIs. At the amino acid level, they share high sequence similarity with each other and with annexins from higher plants. Phylogenetic analysis revealed their evolutionary relationship with corresponding orthologous sequences in Arabidopsis and deduced proteins in various plant species. Expression analysis by semi-quantitative RT-PCR revealed that these genes are differentially expressed in various tissues. The expression patterns of these genes also showed regulation by various stress conditions such as exposure to signaling molecules, salinity and oxidative stress and wounding. Additionally, the in silico promoter analysis (of AnnBj1, AnnBj2 and AnnBj3) showed the presence of different cis-responsive elements that could respond to various stress conditions. These results indicate that AnnBj genes may play important roles in adaptation of plants to various environmental stresses.

AB - Plant annexins constitute a multigene family having suggested roles in a variety of cellular processes including stress responses. We have isolated and characterized five different cDNAs of mustard, Brassica juncea (AnnBj1, AnnBj2, AnnBj3, AnnBj6 and AnnBj7) encoding annexin proteins using a RT-PCR/RACE-PCR based strategy. The predicted molecular masses of these annexins are ∼36.0 kDa with acidic pIs. At the amino acid level, they share high sequence similarity with each other and with annexins from higher plants. Phylogenetic analysis revealed their evolutionary relationship with corresponding orthologous sequences in Arabidopsis and deduced proteins in various plant species. Expression analysis by semi-quantitative RT-PCR revealed that these genes are differentially expressed in various tissues. The expression patterns of these genes also showed regulation by various stress conditions such as exposure to signaling molecules, salinity and oxidative stress and wounding. Additionally, the in silico promoter analysis (of AnnBj1, AnnBj2 and AnnBj3) showed the presence of different cis-responsive elements that could respond to various stress conditions. These results indicate that AnnBj genes may play important roles in adaptation of plants to various environmental stresses.

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KW - Calcium-binding protein

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ER -

Molecular cloning and characterization of five annexin genes from Indian mustard (Brassica juncea L. Czern and Coss)

Abstract

Bibliographical note

Keywords

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