Molecular detection of Anaplasma platys and Ehrlichia canis in dogs from the North of Portugal

Luís Cardoso*, Joana Tuna, Lisete Vieira, Yael Yisaschar-Mekuzas, Gad Baneth

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

38 Scopus citations

Abstract

Tick-transmitted rickettsial pathogens belonging to the Ehrlichia and Anaplasma genera can infect dogs and humans. In this study, four dogs from the North of Portugal, in which an ehrlichial disease was suspected clinically, were tested by molecular methods. After DNA extraction from blood on filter paper, a 345 bp fragment of the Ehrlichia/Anaplasma 16S rRNA gene was amplified by the polymerase chain reaction (PCR). Sequence analysis of PCR products revealed one dog infected with Ehrlichia canis and three with Anaplasma platys. One of these latter animals was co-infected with Babesia canis subspecies vogeli. This is the first report of the genetic characterisation of both A. platys and E. canis in naturally infected dogs from the North of Portugal.

Original languageAmerican English
Pages (from-to)232-233
Number of pages2
JournalVeterinary Journal
Volume183
Issue number2
DOIs
StatePublished - Feb 2010

Keywords

  • Anaplasma platys
  • Canine
  • Ehrlichia canis
  • Polymerase chain reaction
  • Portugal

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