Molecular detection of bartonella species in fleas collected from dogs and cats from Costa Rica

Norman Rojas*, Adriana Troyo, Daniela Castillo, Ricardo Gutierrez, Shimon Harrus

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

8 Scopus citations


The bacterial genus Bartonella includes several species with zoonotic potential, some of which are common in domestic dogs and cats, as well as in their fleas. Because there is no previous information about the presence of Bartonella species in fleas from Central America, this study aimed at evaluating the presence of Bartonella spp. in fleas collected from dogs and cats in Costa Rica. A total 72 pools of Ctenocephalides felis and 21 pools of Pulex simulans were screened by conventional PCR to detect Bartonella DNA fragments of the citrate synthase (gltA) and the β subunit RNA polymerase (rpoB) genes. Three (4.2%) pools of C. felis and five pools (22.7%) of P. simulans were found positive for Bartonella DNA. Sequences corresponding to Bartonella vinsonii subsp. berkhoffii strain Winnie, B. rochalimae, and an undescribed Bartonella sp. (clone BR10) were detected in flea pools from dogs, whereas Bartonella henselae and B. clarridgeiae sequences were identified in flea pools from cats. The detection of zoonotic Bartonella spp. in this study should increase the awareness to these flea-borne diseases among physicians and public health workers and highlight the importance of flea control in the region.

Original languageAmerican English
Pages (from-to)630-632
Number of pages3
JournalVector-Borne and Zoonotic Diseases
Issue number10
StatePublished - 1 Oct 2015

Bibliographical note

Publisher Copyright:
© Mary Ann Liebert, Inc. 2015.


  • Bartonella
  • Bartonellosis
  • Cats
  • Ctenocephalides felis
  • Dogs
  • Domestic animals
  • PCR
  • Pulex


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