TY - JOUR
T1 - Molecular detection of human cytomegalovirus and determination of genotypic ganciclovir resistance in clinical specimens
AU - Spector, Stephen A.
AU - Usia, Karen
AU - Wolf, Dana
AU - Shinkai, Mika
AU - Smith, Irene
PY - 1995/10
Y1 - 1995/10
N2 - The data presented in this article demonstrate how the polymerase chain reaction (PeR) can be used to detect human cytomegalovirus (HCMV) DNA in the plasma and cerebrospinal fluid (CSF) of infected individuals. Detection of HCMVDNA in plasma of transplant recipients and persons infected with human immunodeficiency virus identifies people with acute visceral disease and those at highest risk for development of HCMV disease. The detection of HCMV DNA in CSF is of particular help in identifying persons with HCMV-related central nervous system disease. In addition, specific mutations within the UL97 region, which encodes for a HCMV protein kinase, have been found to confer ganciclovir resistance and can be detected in plasma and CSF by direct sequencing of PeR products. These data will help provide tools for clinicians to better diagnose, manage, and treat persons with HCMV disease.
AB - The data presented in this article demonstrate how the polymerase chain reaction (PeR) can be used to detect human cytomegalovirus (HCMV) DNA in the plasma and cerebrospinal fluid (CSF) of infected individuals. Detection of HCMVDNA in plasma of transplant recipients and persons infected with human immunodeficiency virus identifies people with acute visceral disease and those at highest risk for development of HCMV disease. The detection of HCMV DNA in CSF is of particular help in identifying persons with HCMV-related central nervous system disease. In addition, specific mutations within the UL97 region, which encodes for a HCMV protein kinase, have been found to confer ganciclovir resistance and can be detected in plasma and CSF by direct sequencing of PeR products. These data will help provide tools for clinicians to better diagnose, manage, and treat persons with HCMV disease.
UR - http://www.scopus.com/inward/record.url?scp=0028863049&partnerID=8YFLogxK
U2 - 10.1093/clinids/21.Supplement_2.S170
DO - 10.1093/clinids/21.Supplement_2.S170
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C2 - 8845447
AN - SCOPUS:0028863049
SN - 1058-4838
VL - 21
SP - S170-S173
JO - Clinical Infectious Diseases
JF - Clinical Infectious Diseases
ER -
