Molecular detection of Rickettsia aeschlimannii in Hyalomma spp. ticks from camels (Camelus dromedarius) in Nigeria, West Africa

J. Kamani*, G. Baneth, D. A. Apanaskevich, K. Y. Mumcuoglu, S. Harrus

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

32 Scopus citations

Abstract

Several species of the spotted fever group rickettsiae have been identified as emerging pathogens throughout the world, including in Africa. In this study, 197 Hyalomma ticks (Ixodida: Ixodidae) collected from 51 camels (Camelus dromedarius) in Kano, northern Nigeria, were screened by amplification and sequencing of the citrate synthase (gltA), outer membrane protein A (ompA) and 17-kDa antigen gene fragments. Rickettsia sp. gltA fragments were detected in 43.3% (42/97) of the tick pools tested. Rickettsial ompA gene fragments (189bp and 630bp) were detected in 64.3% (n=27) and 23.8% (n=10) of the gltA-positive tick pools by real-time and conventional polymerase chain reaction (PCR), respectively. The amplicons were 99-100% identical to Rickettsia aeschlimannii TR/Orkun-H and R.aeschlimannii strain EgyRickHimp-El-Arish in GenBank. Furthermore, 17-kDa antigen gene fragments of 214bp and 265bp were detected in 59.5% (n=25) and 38.1% (n=16), respectively, of tick pools, and sequences were identical to one another and 99-100% identical to those of the R.aeschlimannii strain Ibadan A1 in GenBank. None of the Hyalomma impressum ticks collected were positive for Rickettsia sp. DNA. Rickettsia sp. gltA fragments (133bp) were detected in 18.8% of camel blood samples, but all samples were negative for the other genes targeted. This is the first report to describe the molecular detection of R.aeschlimannii in Hyalomma spp. ticks from camels in Nigeria.

Original languageAmerican English
Pages (from-to)205-209
Number of pages5
JournalMedical and Veterinary Entomology
Volume29
Issue number2
DOIs
StatePublished - 1 Jun 2015

Bibliographical note

Publisher Copyright:
© 2015 The Royal Entomological Society.

Keywords

  • Camels
  • Hyalomma ticks
  • Molecular detection
  • Nigeria
  • Rickettsia aeschlimannii

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