Molecular detection of Theileria, Babesia, and Hepatozoon spp. in ixodid ticks from Palestine

Kifaya Azmi*, Suheir Ereqat, Abedelmajeed Nasereddin, Amer Al-Jawabreh, Gad Baneth, Ziad Abdeen

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

22 Scopus citations

Abstract

Ixodid ticks transmit various infectious agents that cause disease in humans and livestock worldwide. A cross-sectional survey on the presence of protozoan pathogens in ticks was carried out to assess the impact of tick-borne protozoa on domestic animals in Palestine. Ticks were collected from herds with sheep, goats and dogs in different geographic districts and their species were determined using morphological keys. The presence of piroplasms and Hepatozoon spp. was determined by PCR amplification of a 460–540 bp fragment of the 18S rRNA gene followed by RFLP or DNA sequencing. A PCR-RFLP method based on the 18S rRNA was used in order to detect and to identify Hepatozoon, Babesia and Theileria spp. A total of 516 ticks were collected from animals in six Palestinian localities. Five tick species were found: Rhipicephalus sanguineus sensu lato, Rhipicephalus turanicus, Rhipicephalus bursa, Haemaphysalis parva and Haemaphysalis adleri. PCR-based analyses of the ticks revealed Theileria ovis (5.4%), Hepatozoon canis (4.3%), Babesia ovis (0.6%), and Babesia vogeli (0.4%). Theileria ovis was significantly associated with ticks from sheep and with R. turanicus ticks (p < 0.01). H. canis was detected only in R. sanguineus s.l. and was significantly associated with ticks from dogs (p < 0.01). To our knowledge, this is the first report describing the presence of these pathogens in ticks collected from Palestine. Communicating these findings with health and veterinary professionals will increase their awareness, and contribute to improved diagnosis and treatment of tick-borne diseases.

Original languageAmerican English
Pages (from-to)734-741
Number of pages8
JournalTicks and Tick-borne Diseases
Volume7
Issue number5
DOIs
StatePublished - 1 Jul 2016

Bibliographical note

Funding Information:
This study was supported financially by grant 2014.52146 funded by the Netherlands Ministry of Foreign Affairs, The Hague, Netherlands and USAID grant MERC TA-MOU-12-M32-038. We thank Mr. Samir Sawalha, Taher Zaid and Ahmad Abdelkader for their kind help during sample collection.

Publisher Copyright:
© 2016

Keywords

  • Babesia
  • Hepatozoon
  • Theileria
  • Ticks

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