Molecular diagnostics for detecting pyrethroid and organophosphate resistance mutations in the Q biotype of the whitefly Bemisia tabaci (Hemiptera: Aleyrodidae)

Anastasia Tsagkarakou*, Dimitra Nikou, Emmanouil Roditakis, Michal Sharvit, Shai Morin, John Vontas

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

41 Scopus citations

Abstract

Pyrethroid and organophosphate resistance-associated mutations have been recently reported in the whitefly Bemisia tabaci (Gennadius), a major pest of protected and outdoor crops worldwide. Here, we developed simple PCR-agarose gel visualization based assays for reliably monitoring the L925I and T929V pyrethroid resistance mutations in the B. tabaci para-type voltage gated sodium channel and the iAChE F331W organophosphate resistance mutation in the acetylcholinesterase enzyme ace1. PCR-RFLP assays were developed for detecting the L925I and the F331W resistance mutations. A highly specific PASA was developed for detecting the T929V mutation. The molecular diagnostic tools were used to monitor the frequency of the resistance mutations in a large number of field caught Q biotype B. tabaci from Crete (Greece), where both organophosphates and pyrethroids are extensively used. The F331W mutation was fixed in all field individuals examined. The pyrethroid resistance mutations were detected in high frequencies: 0.38 and 0.54 for L925I and T929V, respectively. The simple diagnostics are accurate and robust, to be used alongside classical bioassays to prevent ineffective insecticide applications, and for early identification of the spreading of resistant Q biotype populations into new regions around the globe.

Original languageAmerican English
Pages (from-to)49-54
Number of pages6
JournalPesticide Biochemistry and Physiology
Volume94
Issue number2-3
DOIs
StatePublished - Jun 2009

Bibliographical note

Funding Information:
We are grateful to Dr. Ralf Nauen (Bayer Crop Science) for continuous support and suggestions. The project was partially funded by Bayer Hellas Crop Science and GSRT/Bilateral Projects Greece—France (GGET-229-e).

Keywords

  • Acetylcholinesterase
  • Bemisia tabaci
  • Genotype
  • Molecular diagnostics
  • Resistance management
  • kdr

Fingerprint

Dive into the research topics of 'Molecular diagnostics for detecting pyrethroid and organophosphate resistance mutations in the Q biotype of the whitefly Bemisia tabaci (Hemiptera: Aleyrodidae)'. Together they form a unique fingerprint.

Cite this