Molecular manipulations for enhancing luminescent bioreporters performance in the detection of toxic chemicals

Sharon Yagur-Kroll*, Shimshon Belkin

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Microbial whole-cell bioreporters are genetically modified microorganisms that produce a quantifiable output in response to the presence of toxic chemicals or other stress factors. These bioreporters harbor a genetic fusion between a sensing element (usually a gene regulatory element responsive to the target) and a reporter element, the product of which may be quantitatively monitored either by its presence or by its activity. In this chapter we review genetic manipulations undertaken in order to improve bioluminescent bioreporter performance by increasing luminescent output, lowering the limit of detection, and shortening the response time. We describe molecular manipulations applied to all aspects of whole-cell bioreporters: the host strain, the expression system, the sensing element, and the reporter element.

Original languageEnglish
Pages (from-to)137-149
Number of pages13
JournalAdvances in Biochemical Engineering/Biotechnology
Volume145
DOIs
StatePublished - 1 Nov 2014

Bibliographical note

Publisher Copyright:
© Springer-Verlag Berlin Heidelberg 2014.

Keywords

  • Bioluminescence
  • Bioreporters
  • Biosensors
  • Escherichia coli
  • Fluorescence
  • Genetic engineering

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