TY - JOUR
T1 - Molecular manipulations for enhancing luminescent bioreporters performance in the detection of toxic chemicals
AU - Yagur-Kroll, Sharon
AU - Belkin, Shimshon
N1 - Publisher Copyright:
© Springer-Verlag Berlin Heidelberg 2014.
PY - 2014/11/1
Y1 - 2014/11/1
N2 - Microbial whole-cell bioreporters are genetically modified microorganisms that produce a quantifiable output in response to the presence of toxic chemicals or other stress factors. These bioreporters harbor a genetic fusion between a sensing element (usually a gene regulatory element responsive to the target) and a reporter element, the product of which may be quantitatively monitored either by its presence or by its activity. In this chapter we review genetic manipulations undertaken in order to improve bioluminescent bioreporter performance by increasing luminescent output, lowering the limit of detection, and shortening the response time. We describe molecular manipulations applied to all aspects of whole-cell bioreporters: the host strain, the expression system, the sensing element, and the reporter element.
AB - Microbial whole-cell bioreporters are genetically modified microorganisms that produce a quantifiable output in response to the presence of toxic chemicals or other stress factors. These bioreporters harbor a genetic fusion between a sensing element (usually a gene regulatory element responsive to the target) and a reporter element, the product of which may be quantitatively monitored either by its presence or by its activity. In this chapter we review genetic manipulations undertaken in order to improve bioluminescent bioreporter performance by increasing luminescent output, lowering the limit of detection, and shortening the response time. We describe molecular manipulations applied to all aspects of whole-cell bioreporters: the host strain, the expression system, the sensing element, and the reporter element.
KW - Bioluminescence
KW - Bioreporters
KW - Biosensors
KW - Escherichia coli
KW - Fluorescence
KW - Genetic engineering
UR - http://www.scopus.com/inward/record.url?scp=84921632074&partnerID=8YFLogxK
U2 - 10.1007/978-3-662-43619-6_4
DO - 10.1007/978-3-662-43619-6_4
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C2 - 25216954
AN - SCOPUS:84921632074
SN - 0724-6145
VL - 145
SP - 137
EP - 149
JO - Advances in Biochemical Engineering/Biotechnology
JF - Advances in Biochemical Engineering/Biotechnology
ER -