TY - JOUR
T1 - Molecular weight, shape and structure of mixed micelles of Triton X-100 and sphingomyelin
AU - Yedgar, S.
AU - Barenholz, Y.
AU - Cooper, V. G.
PY - 1974/8/21
Y1 - 1974/8/21
N2 - The sedimentation coefficients of mixed micelles of Triton X-100 and sphingomyelin have been measured for Triton molar fractions between 0.32 and 0.79. These measurements, together with previously reported diffusion coefficients, are used to calculate the molecular weights of the mixed micelles, and their aggregation numbers. It is found that the molecular weight decreases steadily with increasing Triton molar fraction. However, the Triton aggregation number remains nearly constant at about 196, so that the decreasing molecular weight is reflected in the sphingomyelin aggregation number, which decreases from 442 to 50 as the Triton molar fraction increases from 0.32-0.79. Intrinsic viscosities of the mixed micelle solutions are found to be independent of Triton molar fractions, with a value of 5.5 ml/g. From these measurements a number of inferences are drawn regarding the micellar shape and structure.
AB - The sedimentation coefficients of mixed micelles of Triton X-100 and sphingomyelin have been measured for Triton molar fractions between 0.32 and 0.79. These measurements, together with previously reported diffusion coefficients, are used to calculate the molecular weights of the mixed micelles, and their aggregation numbers. It is found that the molecular weight decreases steadily with increasing Triton molar fraction. However, the Triton aggregation number remains nearly constant at about 196, so that the decreasing molecular weight is reflected in the sphingomyelin aggregation number, which decreases from 442 to 50 as the Triton molar fraction increases from 0.32-0.79. Intrinsic viscosities of the mixed micelle solutions are found to be independent of Triton molar fractions, with a value of 5.5 ml/g. From these measurements a number of inferences are drawn regarding the micellar shape and structure.
UR - http://www.scopus.com/inward/record.url?scp=0016249394&partnerID=8YFLogxK
U2 - 10.1016/0005-2736(74)90009-1
DO - 10.1016/0005-2736(74)90009-1
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C2 - 4853249
AN - SCOPUS:0016249394
SN - 0005-2736
VL - 363
SP - 98
EP - 111
JO - Biochimica et Biophysica Acta - Biomembranes
JF - Biochimica et Biophysica Acta - Biomembranes
IS - 1
ER -