Moloney lymphoma cells express a polyprotein contanining the GAG gene‐coded p15 and the moloney leukemia virus‐induced cell surface antigen (MCSA)

Anjali Karande*, Eitan Yefenof, Eva Maria Fenyö, George Klein

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

The relationship between MCSA and the viral structural proteins in YAC Moloney lymphoma cells was further investigated by using membrane immunofluorescence and immunoadsorbent columns. As previously observed, MCSA showed only minimal capping and thereby differed In behaviour from gp70, p30 and p12 virion antigens. Whereas antibody‐induced capping of gp70 did not change the membrane distribution of MCSA, co‐capping was observed between MCSA and p30 and p12 gag protein antigens. This indicated that, whereas MCSA is distinct from gp70, it is linked to p30 and p12 on the membrane of living cells. It was then attempted to isolate MCSA on anti‐p30 and anti‐pIS immunoadsorbent columns from solubilized YAC cells. Contrary to what was expected, no MCSA was bound to the anti‐p30 column. On the other hand, a part (10–15%) of the MCSA was bound to the anti‐pIS column, indicating that MCSA is linked to the gag p15.

Original languageEnglish
Pages (from-to)508-513
Number of pages6
JournalInternational Journal of Cancer
Volume23
Issue number4
DOIs
StatePublished - 15 Apr 1979

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