Monocyte and neutrophil isolation and migration assays

Simon Yona*, Richard Hayhoe, Inbal Avraham-Davidi

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review

19 Scopus citations


This unit describes methods for isolating mouse monocytes and neutrophils, as well as in vitro protocols for measuring cell migration and polarization. The method employed here for the isolation of nave phagocytes overcomes many of the difficulties previously encountered concerning phagocyte activation. Three in vitro protocols are provided for the analysis of cell migration, one requiring no specialized equipment, one requiring the modified Boyden chamber, and the other employing a flow chamber, which measures cell adhesion, rolling, and migration. Finally, a method is provided for imaging polarized cells by confocal microscopy.

Original languageAmerican English
Pages (from-to)14.15.1-14.15.14
JournalCurrent Protocols in Immunology
Issue numberSUPPL. 88
StatePublished - 2010
Externally publishedYes


  • Cell polarization
  • Chemotaxis
  • Flow chamber
  • Monocyte
  • Neutrophil


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