TY - JOUR
T1 - Monocyte and neutrophil isolation and migration assays
AU - Yona, Simon
AU - Hayhoe, Richard
AU - Avraham-Davidi, Inbal
PY - 2010
Y1 - 2010
N2 - This unit describes methods for isolating mouse monocytes and neutrophils, as well as in vitro protocols for measuring cell migration and polarization. The method employed here for the isolation of nave phagocytes overcomes many of the difficulties previously encountered concerning phagocyte activation. Three in vitro protocols are provided for the analysis of cell migration, one requiring no specialized equipment, one requiring the modified Boyden chamber, and the other employing a flow chamber, which measures cell adhesion, rolling, and migration. Finally, a method is provided for imaging polarized cells by confocal microscopy.
AB - This unit describes methods for isolating mouse monocytes and neutrophils, as well as in vitro protocols for measuring cell migration and polarization. The method employed here for the isolation of nave phagocytes overcomes many of the difficulties previously encountered concerning phagocyte activation. Three in vitro protocols are provided for the analysis of cell migration, one requiring no specialized equipment, one requiring the modified Boyden chamber, and the other employing a flow chamber, which measures cell adhesion, rolling, and migration. Finally, a method is provided for imaging polarized cells by confocal microscopy.
KW - Cell polarization
KW - Chemotaxis
KW - Flow chamber
KW - Monocyte
KW - Neutrophil
UR - http://www.scopus.com/inward/record.url?scp=77949343135&partnerID=8YFLogxK
U2 - 10.1002/0471142735.im1415s88
DO - 10.1002/0471142735.im1415s88
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C2 - 20143313
AN - SCOPUS:77949343135
SN - 1934-3671
SP - 14.15.1-14.15.14
JO - Current Protocols in Immunology
JF - Current Protocols in Immunology
IS - SUPPL. 88
ER -