Monocyte and neutrophil isolation and migration assays

Simon Yona; Richard Hayhoe; Inbal Avraham-Davidi

doi:10.1002/0471142735.im1415s88

Monocyte and neutrophil isolation and migration assays

Simon Yona^*, Richard Hayhoe, Inbal Avraham-Davidi

^*Corresponding author for this work

Research output: Contribution to journal › Review article › peer-review

19 Scopus citations

Abstract

This unit describes methods for isolating mouse monocytes and neutrophils, as well as in vitro protocols for measuring cell migration and polarization. The method employed here for the isolation of nave phagocytes overcomes many of the difficulties previously encountered concerning phagocyte activation. Three in vitro protocols are provided for the analysis of cell migration, one requiring no specialized equipment, one requiring the modified Boyden chamber, and the other employing a flow chamber, which measures cell adhesion, rolling, and migration. Finally, a method is provided for imaging polarized cells by confocal microscopy.

Original language	American English
Pages (from-to)	14.15.1-14.15.14
Journal	Current Protocols in Immunology
Issue number	SUPPL. 88
DOIs	https://doi.org/10.1002/0471142735.im1415s88
State	Published - 2010
Externally published	Yes

Keywords

Cell polarization
Chemotaxis
Flow chamber
Monocyte
Neutrophil

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title = "Monocyte and neutrophil isolation and migration assays",

abstract = "This unit describes methods for isolating mouse monocytes and neutrophils, as well as in vitro protocols for measuring cell migration and polarization. The method employed here for the isolation of nave phagocytes overcomes many of the difficulties previously encountered concerning phagocyte activation. Three in vitro protocols are provided for the analysis of cell migration, one requiring no specialized equipment, one requiring the modified Boyden chamber, and the other employing a flow chamber, which measures cell adhesion, rolling, and migration. Finally, a method is provided for imaging polarized cells by confocal microscopy.",

keywords = "Cell polarization, Chemotaxis, Flow chamber, Monocyte, Neutrophil",

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T1 - Monocyte and neutrophil isolation and migration assays

AU - Yona, Simon

AU - Hayhoe, Richard

AU - Avraham-Davidi, Inbal

PY - 2010

Y1 - 2010

N2 - This unit describes methods for isolating mouse monocytes and neutrophils, as well as in vitro protocols for measuring cell migration and polarization. The method employed here for the isolation of nave phagocytes overcomes many of the difficulties previously encountered concerning phagocyte activation. Three in vitro protocols are provided for the analysis of cell migration, one requiring no specialized equipment, one requiring the modified Boyden chamber, and the other employing a flow chamber, which measures cell adhesion, rolling, and migration. Finally, a method is provided for imaging polarized cells by confocal microscopy.

AB - This unit describes methods for isolating mouse monocytes and neutrophils, as well as in vitro protocols for measuring cell migration and polarization. The method employed here for the isolation of nave phagocytes overcomes many of the difficulties previously encountered concerning phagocyte activation. Three in vitro protocols are provided for the analysis of cell migration, one requiring no specialized equipment, one requiring the modified Boyden chamber, and the other employing a flow chamber, which measures cell adhesion, rolling, and migration. Finally, a method is provided for imaging polarized cells by confocal microscopy.

KW - Cell polarization

KW - Chemotaxis

KW - Flow chamber

KW - Monocyte

KW - Neutrophil

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U2 - 10.1002/0471142735.im1415s88

DO - 10.1002/0471142735.im1415s88

M3 - Review article

C2 - 20143313

AN - SCOPUS:77949343135

SN - 1934-3671

SP - 14.15.1-14.15.14

JO - Current Protocols in Immunology

JF - Current Protocols in Immunology

IS - SUPPL. 88

ER -

Monocyte and neutrophil isolation and migration assays

Abstract

Keywords

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