Monocyte and Neutrophil Isolation, Migration, and Phagocytosis Assays

Alexander Maini; Juliet R. Foote; Richard Hayhoe; Amit A. Patel; Alastair O'Brien; Inbal Avraham-Davidi; Simon Yona

doi:10.1002/cpim.53

Monocyte and Neutrophil Isolation, Migration, and Phagocytosis Assays

Alexander Maini, Juliet R. Foote, Richard Hayhoe, Amit A. Patel, Alastair O'Brien, Inbal Avraham-Davidi, Simon Yona

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

This article describes methods for isolating mouse monocytes and neutrophils, as well as in vitro protocols for measuring cell phagocytosis, migration, and polarization. The method employed here for the isolation of naive phagocytes overcomes many of the difficulties previously encountered concerning phagocyte activation. Three in vitro protocols are provided for the analysis of cell migration, one requiring no specialized equipment, one requiring a modified Boyden chamber, and the other employing a flow chamber, which measures cell adhesion, rolling, and migration. Three in vitro protocols to examine phagocytosis have been included in this updated version. Finally, a method is provided for imaging polarized cells by confocal microscopy.

Original language	English
Pages (from-to)	e53
Journal	Current Protocols in Immunology
Volume	122
Issue number	1
DOIs	https://doi.org/10.1002/cpim.53
State	Published - 1 Aug 2018
Externally published	Yes

Bibliographical note

Publisher Copyright:
© 2018 John Wiley & Sons, Inc.

Keywords

cell polarization
chemotaxis
flow chamber
monocyte
neutrophil
phagocytosis

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Monocyte and Neutrophil Isolation, Migration, and Phagocytosis Assays

Abstract

Bibliographical note

Keywords

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