Monoterpene glycoside biosynthesis in detached grape berries grown in vitro

B. Bravdo*, O. Shoseyov, R. Ikan, A. Altman

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

A procedure for the culture in vitro of isolated small berries of Vitis vinifera L. cv. Muscat of Alexandria in a Murashige and Skoog basal medium supplemented with N6‐benzyladenine and indoleacetic acid is described. Berries developed well in culture during 60 days and tripled in size, but remained green and smaller than normal berries grown in vivo. Some callus formed on the distal end of the berry, and where major skin damage occurred, callus emerged from the cracked berries. In order to examine their biosynthetic competency, berries which were previously cultured in vitro for 60 days were incubated for 48 h in a Murashige and Skoog medium containing a [14C]‐labelled water‐soluble fraction. This fraction was isolated from grape berries located adjacent to a leaf that had been exposed to gaseous 14CO2 in full sunlight for 5 h. The berries were then recultured for 48 h after which a glycosidic fraction was isolated on a C18 reversed phase column and further separated by thin layer chromatography (TLC). The major labelled band corresponded to the geranyl‐β‐rutinoside marker, indicating that grape berries have the ability to synthesize monoterpene glycosides. This band also consisted of other monoterpene glycosides as revealed by the gas chromatography‐mass spectrometry (GC‐MS) analysis of their aglycones (released by enzymatic hydrolysis).

Original languageAmerican English
Pages (from-to)93-99
Number of pages7
JournalPhysiologia Plantarum
Volume78
Issue number1
DOIs
StatePublished - Jan 1990

Keywords

  • Berries
  • N‐benzyladenine
  • Vitis vinifera
  • biosynthesis
  • carbon dioxide
  • glycosides
  • grape
  • in vitro culture
  • indoleacetic acid
  • monoterpene

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