MPST but not CSE is the primary regulator of hydrogen sulfide production and function in the coronary artery

Maggie M. Kuo, Dae Hee Kim, Sandeep Jandu, Yehudit Bergman, Siqi Tan, Huilei Wang, Deepesh R. Pandey, Theodore P. Abraham, Artin A. Shoukas, Dan E. Berkowitz, Lakshmi Santhanam*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

46 Scopus citations


Hydrogen sulfide (H2S) has emerged as an important gasotransmitter in the vasculature. In this study, we tested the hypothesis that H2S contributes to coronary vasoregulation and evaluated the physiological relevance of two sources of H2S, namely, cystathionine-γ-lyase (CSE) and 3-mercaptypyruvate sulfertransferase (MPST). MPST was detected in human coronary artery endothelial cells as well as rat and mouse coronary artery; CSE was not detected in the coronary vasculature. Rat coronary artery homogenates produced H2S through the MPST pathway but not the CSE pathway in vitro. In vivo coronary vasorelaxation response was similar in CSE knockout mice, wild-type mice (WT), and WT mice treated with the CSE inhibitor propargylglycine, suggesting that CSE-produced H2S does not have a significant role in coronary vasoregulation in vivo. Ex vivo, the MPST substrate 3-mercaptopyruvate (3-MP) and H2S donor sodium hydrosulfide (NaHS) elicited similar coronary vasoreactivity responses. Pyruvate did not have any effects on vasoreactivity. The vasoactive effect of H2S appeared to be nitric oxide (NO) dependent: H2S induced coronary vasoconstriction in the presence of NO and vasorelaxation in its absence. Maximal endothelial-dependent relaxation was intact after 3-MP and NaHS induced an increase in preconstriction tone, suggesting that endothelial NO synthase activity was not significantly inhibited. In vitro, H2S reacted with NO, which may, in part explain the vasoconstrictive effects of 3-MP and NaHS. Taken together, these data show that MPST rather than CSE generates H2S in coronary artery, mediating its effects through direct modulation of NO. This has important implications for H2S-based therapy in healthy and diseased coronary arteries.

Original languageAmerican English
Pages (from-to)H71-H79
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Issue number1
StatePublished - Jan 2016
Externally publishedYes

Bibliographical note

Publisher Copyright:
© 2016 the American Physiological Society.


  • 3-mercaptopyruvate sulfurtransferase
  • Coronary tone
  • Coronary vasoregulation
  • Cystathionine-γ-lyase
  • hydrogen sulfide


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