Multi-center evaluation of one commercial and 12 in-house real-time PCR assays for detection of Mycoplasma pneumoniae

on behalf of ESCMID Study Group for Mycoplasma Infections

doi:10.1016/j.diagmicrobio.2017.03.004

Multi-center evaluation of one commercial and 12 in-house real-time PCR assays for detection of Mycoplasma pneumoniae

on behalf of ESCMID Study Group for Mycoplasma Infections

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

Detection of Mycoplasma pneumoniae by real-time PCR is not yet standardized across laboratories. We have implemented a standardization protocol to compare the performance of thirteen commercial and in-house approaches. Despite differences on threshold values of samples, all assays were able to detect at least 20 M. pneumoniae genomes per reaction.

Original language	English
Pages (from-to)	111-114
Number of pages	4
Journal	Diagnostic Microbiology and Infectious Disease
Volume	88
Issue number	2
DOIs	https://doi.org/10.1016/j.diagmicrobio.2017.03.004
State	Published - 1 Jun 2017
Externally published	Yes

Bibliographical note

Publisher Copyright:
© 2017 Elsevier Inc.

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Detection
Multi-center evaluation
Mycoplasma pneumoniae
Real-time PCR

Access to Document

10.1016/j.diagmicrobio.2017.03.004

Cite this

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title = "Multi-center evaluation of one commercial and 12 in-house real-time PCR assays for detection of Mycoplasma pneumoniae",

abstract = "Detection of Mycoplasma pneumoniae by real-time PCR is not yet standardized across laboratories. We have implemented a standardization protocol to compare the performance of thirteen commercial and in-house approaches. Despite differences on threshold values of samples, all assays were able to detect at least 20 M. pneumoniae genomes per reaction.",

keywords = "Detection, Multi-center evaluation, Mycoplasma pneumoniae, Real-time PCR",

author = "\{on behalf of ESCMID Study Group for Mycoplasma Infections\} and Roger Dumke and Benitez, \{Alvaro J.\} and Victoria Chalker and Karolina Gullsby and Birgit Henrich and Carlos Hidalgo-Grass and Theo Hoogenboezem and Darja Kese and Katherine Loens and Jolanda Maaskant and Ayelet Michael-Gayego and Moses, \{Allon E.\} and Ran Nir-Paz and Pas, \{Suzan D.\} and Sabine Pereyre and Petersen, \{Randi F.\} and Meike Rosenblatt and \{van Rossum\}, \{Annemarie M.C.\} and Uldum, \{S{\o}ren A.\} and Unger, \{Wendy W.J.\} and Dominique Ursi and Winchell, \{Jonas M.\} and Cecile Bebear",

note = "Publisher Copyright: {\textcopyright} 2017 Elsevier Inc.",

year = "2017",

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doi = "10.1016/j.diagmicrobio.2017.03.004",

language = "אנגלית",

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T1 - Multi-center evaluation of one commercial and 12 in-house real-time PCR assays for detection of Mycoplasma pneumoniae

AU - on behalf of ESCMID Study Group for Mycoplasma Infections

AU - Dumke, Roger

AU - Benitez, Alvaro J.

AU - Chalker, Victoria

AU - Gullsby, Karolina

AU - Henrich, Birgit

AU - Hidalgo-Grass, Carlos

AU - Hoogenboezem, Theo

AU - Kese, Darja

AU - Loens, Katherine

AU - Maaskant, Jolanda

AU - Michael-Gayego, Ayelet

AU - Moses, Allon E.

AU - Nir-Paz, Ran

AU - Pas, Suzan D.

AU - Pereyre, Sabine

AU - Petersen, Randi F.

AU - Rosenblatt, Meike

AU - van Rossum, Annemarie M.C.

AU - Uldum, Søren A.

AU - Unger, Wendy W.J.

AU - Ursi, Dominique

AU - Winchell, Jonas M.

AU - Bebear, Cecile

PY - 2017/6/1

Y1 - 2017/6/1

N2 - Detection of Mycoplasma pneumoniae by real-time PCR is not yet standardized across laboratories. We have implemented a standardization protocol to compare the performance of thirteen commercial and in-house approaches. Despite differences on threshold values of samples, all assays were able to detect at least 20 M. pneumoniae genomes per reaction.

AB - Detection of Mycoplasma pneumoniae by real-time PCR is not yet standardized across laboratories. We have implemented a standardization protocol to compare the performance of thirteen commercial and in-house approaches. Despite differences on threshold values of samples, all assays were able to detect at least 20 M. pneumoniae genomes per reaction.

KW - Detection

KW - Multi-center evaluation

KW - Mycoplasma pneumoniae

KW - Real-time PCR

UR - https://www.scopus.com/pages/publications/85015297699

U2 - 10.1016/j.diagmicrobio.2017.03.004

DO - 10.1016/j.diagmicrobio.2017.03.004

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C2 - 28318608

AN - SCOPUS:85015297699

SN - 0732-8893

VL - 88

SP - 111

EP - 114

JO - Diagnostic Microbiology and Infectious Disease

JF - Diagnostic Microbiology and Infectious Disease

IS - 2

ER -

Multi-center evaluation of one commercial and 12 in-house real-time PCR assays for detection of Mycoplasma pneumoniae

Abstract

Bibliographical note

UN SDGs

Keywords

Access to Document

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Cite this