Abstract
The role of different cell types and their interactions in Alzheimer’s disease (AD) is a complex and open question. Here, we pursued this question by assembling a high-resolution cellular map of the aging frontal cortex using single-nucleus RNA sequencing of 24 individuals with a range of clinicopathologic characteristics. We used this map to infer the neocortical cellular architecture of 638 individuals profiled by bulk RNA sequencing, providing the sample size necessary for identifying statistically robust associations. We uncovered diverse cell populations associated with AD, including a somatostatin inhibitory neuronal subtype and oligodendroglial states. We further identified a network of multicellular communities, each composed of coordinated subpopulations of neuronal, glial and endothelial cells, and we found that two of these communities are altered in AD. Finally, we used mediation analyses to prioritize cellular changes that might contribute to cognitive decline. Thus, our deconstruction of the aging neocortex provides a roadmap for evaluating the cellular microenvironments underlying AD and dementia.
Original language | American English |
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Pages (from-to) | 1267-1280 |
Number of pages | 14 |
Journal | Nature Neuroscience |
Volume | 26 |
Issue number | 7 |
DOIs | |
State | Published - Jul 2023 |
Bibliographical note
Funding Information:We thank the individuals who have generously donated their brain to research through the RUSH University Alzheimer’s Disease Center. This work was supported by the National Institute of Aging (NIA), grant nos. AMP-AD U01AG046152, U01AG061356 and RF1AG036042 (P.L.D. and D.A.B); grant nos. R01AG066831 and R21AG075754 and SenNet (grant no. U54AG076040) (V.M.); grant nos. P30AG10161, P30AG72975, R01AG15819 and R01AG17917 (ROSMAP, P.L.D.); the Chan Zuckerberg Initiative (grant no. CS-02018-191971) (P.L.D. and V.M.); Israel Science Foundation (ISF) grant no. 1709/19 (N.H.); the European Research Council grant no. 853409 (N.H.); grant no. MOST/IL 3-15687 (N.H.); and the Klarman Cell Observatory (A.R.). N.H. is a Goren Khazzam senior lecturer in neuroscience and is support by the Myers Foundation. F.Z. and A.R. are investigators of the Howard Hughes Medical Institute.
Funding Information:
We thank the individuals who have generously donated their brain to research through the RUSH University Alzheimer’s Disease Center. This work was supported by the National Institute of Aging (NIA), grant nos. AMP-AD U01AG046152, U01AG061356 and RF1AG036042 (P.L.D. and D.A.B); grant nos. R01AG066831 and R21AG075754 and SenNet (grant no. U54AG076040) (V.M.); grant nos. P30AG10161, P30AG72975, R01AG15819 and R01AG17917 (ROSMAP, P.L.D.); the Chan Zuckerberg Initiative (grant no. CS-02018-191971) (P.L.D. and V.M.); Israel Science Foundation (ISF) grant no. 1709/19 (N.H.); the European Research Council grant no. 853409 (N.H.); grant no. MOST/IL 3-15687 (N.H.); and the Klarman Cell Observatory (A.R.). N.H. is a Goren Khazzam senior lecturer in neuroscience and is support by the Myers Foundation. F.Z. and A.R. are investigators of the Howard Hughes Medical Institute.
Publisher Copyright:
© 2023, The Author(s), under exclusive licence to Springer Nature America, Inc.