Multicellular communities are perturbed in the aging human brain and Alzheimer’s disease

Anael Cain; Mariko Taga; Cristin McCabe; Gilad S. Green; Idan Hekselman; Charles C. White; Dylan I. Lee; Pallavi Gaur; Orit Rozenblatt-Rosen; Feng Zhang; Esti Yeger-Lotem; David A. Bennett; Hyun Sik Yang; Aviv Regev; Vilas Menon; Naomi Habib; Philip L. De Jager

doi:10.1038/s41593-023-01356-x

Multicellular communities are perturbed in the aging human brain and Alzheimer’s disease

Anael Cain, Mariko Taga, Cristin McCabe, Gilad S. Green, Idan Hekselman, Charles C. White, Dylan I. Lee, Pallavi Gaur, Orit Rozenblatt-Rosen, Feng Zhang, Esti Yeger-Lotem, David A. Bennett, Hyun Sik Yang, Aviv Regev, Vilas Menon^*, Naomi Habib^*, Philip L. De Jager^*

^*Corresponding author for this work

Edmond & Lily Safra Center for Brain Sciences

Research output: Contribution to journal › Article › peer-review

22 Scopus citations

Abstract

The role of different cell types and their interactions in Alzheimer’s disease (AD) is a complex and open question. Here, we pursued this question by assembling a high-resolution cellular map of the aging frontal cortex using single-nucleus RNA sequencing of 24 individuals with a range of clinicopathologic characteristics. We used this map to infer the neocortical cellular architecture of 638 individuals profiled by bulk RNA sequencing, providing the sample size necessary for identifying statistically robust associations. We uncovered diverse cell populations associated with AD, including a somatostatin inhibitory neuronal subtype and oligodendroglial states. We further identified a network of multicellular communities, each composed of coordinated subpopulations of neuronal, glial and endothelial cells, and we found that two of these communities are altered in AD. Finally, we used mediation analyses to prioritize cellular changes that might contribute to cognitive decline. Thus, our deconstruction of the aging neocortex provides a roadmap for evaluating the cellular microenvironments underlying AD and dementia.

Original language	English
Pages (from-to)	1267-1280
Number of pages	14
Journal	Nature Neuroscience
Volume	26
Issue number	7
DOIs	https://doi.org/10.1038/s41593-023-01356-x
State	Published - Jul 2023

Bibliographical note

Publisher Copyright:
© 2023, The Author(s), under exclusive licence to Springer Nature America, Inc.

Access to Document

10.1038/s41593-023-01356-x

Cite this

Cain, A., Taga, M., McCabe, C., Green, G. S., Hekselman, I., White, C. C., Lee, D. I., Gaur, P., Rozenblatt-Rosen, O., Zhang, F., Yeger-Lotem, E., Bennett, D. A., Yang, H. S., Regev, A., Menon, V., Habib, N., & De Jager, P. L. (2023). Multicellular communities are perturbed in the aging human brain and Alzheimer’s disease. Nature Neuroscience, 26(7), 1267-1280. https://doi.org/10.1038/s41593-023-01356-x

@article{3259e93fe543498a855f8dc84b611fb1,

title = "Multicellular communities are perturbed in the aging human brain and Alzheimer{\textquoteright}s disease",

abstract = "The role of different cell types and their interactions in Alzheimer{\textquoteright}s disease (AD) is a complex and open question. Here, we pursued this question by assembling a high-resolution cellular map of the aging frontal cortex using single-nucleus RNA sequencing of 24 individuals with a range of clinicopathologic characteristics. We used this map to infer the neocortical cellular architecture of 638 individuals profiled by bulk RNA sequencing, providing the sample size necessary for identifying statistically robust associations. We uncovered diverse cell populations associated with AD, including a somatostatin inhibitory neuronal subtype and oligodendroglial states. We further identified a network of multicellular communities, each composed of coordinated subpopulations of neuronal, glial and endothelial cells, and we found that two of these communities are altered in AD. Finally, we used mediation analyses to prioritize cellular changes that might contribute to cognitive decline. Thus, our deconstruction of the aging neocortex provides a roadmap for evaluating the cellular microenvironments underlying AD and dementia.",

author = "Anael Cain and Mariko Taga and Cristin McCabe and Green, {Gilad S.} and Idan Hekselman and White, {Charles C.} and Lee, {Dylan I.} and Pallavi Gaur and Orit Rozenblatt-Rosen and Feng Zhang and Esti Yeger-Lotem and Bennett, {David A.} and Yang, {Hyun Sik} and Aviv Regev and Vilas Menon and Naomi Habib and {De Jager}, {Philip L.}",

note = "Publisher Copyright: {\textcopyright} 2023, The Author(s), under exclusive licence to Springer Nature America, Inc.",

year = "2023",

month = jul,

doi = "10.1038/s41593-023-01356-x",

language = "אנגלית",

volume = "26",

pages = "1267--1280",

journal = "Nature Neuroscience",

issn = "1097-6256",

publisher = "Nature Publishing Group",

number = "7",

}

Cain, A, Taga, M, McCabe, C, Green, GS, Hekselman, I, White, CC, Lee, DI, Gaur, P, Rozenblatt-Rosen, O, Zhang, F, Yeger-Lotem, E, Bennett, DA, Yang, HS, Regev, A, Menon, V, Habib, N & De Jager, PL 2023, 'Multicellular communities are perturbed in the aging human brain and Alzheimer’s disease', Nature Neuroscience, vol. 26, no. 7, pp. 1267-1280. https://doi.org/10.1038/s41593-023-01356-x

TY - JOUR

T1 - Multicellular communities are perturbed in the aging human brain and Alzheimer’s disease

AU - Cain, Anael

AU - Taga, Mariko

AU - McCabe, Cristin

AU - Green, Gilad S.

AU - Hekselman, Idan

AU - White, Charles C.

AU - Lee, Dylan I.

AU - Gaur, Pallavi

AU - Rozenblatt-Rosen, Orit

AU - Zhang, Feng

AU - Yeger-Lotem, Esti

AU - Bennett, David A.

AU - Yang, Hyun Sik

AU - Regev, Aviv

AU - Menon, Vilas

AU - Habib, Naomi

AU - De Jager, Philip L.

PY - 2023/7

Y1 - 2023/7

N2 - The role of different cell types and their interactions in Alzheimer’s disease (AD) is a complex and open question. Here, we pursued this question by assembling a high-resolution cellular map of the aging frontal cortex using single-nucleus RNA sequencing of 24 individuals with a range of clinicopathologic characteristics. We used this map to infer the neocortical cellular architecture of 638 individuals profiled by bulk RNA sequencing, providing the sample size necessary for identifying statistically robust associations. We uncovered diverse cell populations associated with AD, including a somatostatin inhibitory neuronal subtype and oligodendroglial states. We further identified a network of multicellular communities, each composed of coordinated subpopulations of neuronal, glial and endothelial cells, and we found that two of these communities are altered in AD. Finally, we used mediation analyses to prioritize cellular changes that might contribute to cognitive decline. Thus, our deconstruction of the aging neocortex provides a roadmap for evaluating the cellular microenvironments underlying AD and dementia.

AB - The role of different cell types and their interactions in Alzheimer’s disease (AD) is a complex and open question. Here, we pursued this question by assembling a high-resolution cellular map of the aging frontal cortex using single-nucleus RNA sequencing of 24 individuals with a range of clinicopathologic characteristics. We used this map to infer the neocortical cellular architecture of 638 individuals profiled by bulk RNA sequencing, providing the sample size necessary for identifying statistically robust associations. We uncovered diverse cell populations associated with AD, including a somatostatin inhibitory neuronal subtype and oligodendroglial states. We further identified a network of multicellular communities, each composed of coordinated subpopulations of neuronal, glial and endothelial cells, and we found that two of these communities are altered in AD. Finally, we used mediation analyses to prioritize cellular changes that might contribute to cognitive decline. Thus, our deconstruction of the aging neocortex provides a roadmap for evaluating the cellular microenvironments underlying AD and dementia.

UR - http://www.scopus.com/inward/record.url?scp=85161979052&partnerID=8YFLogxK

U2 - 10.1038/s41593-023-01356-x

DO - 10.1038/s41593-023-01356-x

M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???

C2 - 37336975

AN - SCOPUS:85161979052

SN - 1097-6256

VL - 26

SP - 1267

EP - 1280

JO - Nature Neuroscience

JF - Nature Neuroscience

IS - 7

ER -

Multicellular communities are perturbed in the aging human brain and Alzheimer’s disease

Abstract

Bibliographical note

Access to Document

Other files and links

Fingerprint

Cite this