Multienzyme Inkjet Printed 2D Arrays

Efrat Gdor, Shay Shemesh, Shlomo Magdassi*, Daniel Mandler

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

The use of printing to produce 2D arrays is well established, and should be relatively facile to adapt for the purpose of printing biomaterials; however, very few studies have been published using enzyme solutions as inks. Among the printing technologies, inkjet printing is highly suitable for printing biomaterials and specifically enzymes, as it offers many advantages. Formulation of the inkjet inks is relatively simple and can be adjusted to a variety of biomaterials, while providing nonharmful environment to the enzymes. Here we demonstrate the applicability of inkjet printing for patterning multiple enzymes in a predefined array in a very straightforward, noncontact method. Specifically, various arrays of the enzymes glucose oxidase (GOx), invertase (INV) and horseradish peroxidase (HP) were printed on aminated glass surfaces, followed by immobilization using glutardialdehyde after printing. Scanning electrochemical microscopy (SECM) was used for imaging the printed patterns and to ascertain the enzyme activity. The successful formation of 2D arrays consisting of enzymes was explored as a means of developing the first surface confined enzyme based logic gates. Principally, XOR and AND gates, each consisting of two enzymes as the Boolean operators, were assembled, and their operation was studied by SECM.

Original languageAmerican English
Pages (from-to)17985-17992
Number of pages8
JournalACS applied materials & interfaces
Volume7
Issue number32
DOIs
StatePublished - 19 Aug 2015

Bibliographical note

Publisher Copyright:
© 2015 American Chemical Society.

Keywords

  • biomolecular logic gates
  • enzymes
  • patterning
  • printing
  • scanning electrochemical microscopy

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