TY - JOUR
T1 - Multifarious characterization of Leishmania tropica from a Judean desert focus, exposing intraspecific diversity and incriminating Phlebotomus sergenti as its vector
AU - Schnur, Lionel F.
AU - Nasereddin, Abdelmageed
AU - Eisenberger, Carol L.
AU - Jaffe, Charles L.
AU - El Fari, Mustafa
AU - Azmi, Kifayia
AU - Anders, Gerlind
AU - Killick-Kendrick, Mireille
AU - Killick-Kendrick, Robert
AU - Dedet, Jean Paul
AU - Pratlong, Francine
AU - Kanaan, Moien
AU - Grossman, Tamar
AU - Jacobson, Raymond L.
AU - Schonian, Gabrielle
AU - Warburg, Alon
PY - 2004/4
Y1 - 2004/4
N2 - The predominant sand fly species collected inside houses in Kfar Adumim, an Israeli village in the Judean Desert that is a focus of cutaneous leishmaniasis, was Phlebotomus papatasi, which was also caught attempting to bite humans. Phlebotomus sergenti, which is rarely seen inside houses, constituted the predominant sand fly species in caves near the village. Leishmania isolates from Ph. sergenti and humans typed as Leishmania tropica. Sand fly and human isolates produced similar small nodular cutaneous lesions in hamsters. Isolates produced excreted factor (EF) of subserotypes A9 or A9B2, characteristic of L. tropica and reacted with L. tropica-specific monoclonal antibodies. Isoenzyme analysis consigned the strains to the L. tropica zymodemes MON-137 and MON-275. Molecular genetic analyses confirmed the strains were L. tropica and intraspecific microheterogeneity was observed. Genomic fingerprinting using a mini-satellite probe separated the L. tropica strains into two clusters that were not entirely congruent with geographic distribution. These results support the heterogeneous nature of L. tropica and incriminate Ph. sergenti as its vector in this Judean Desert focus.
AB - The predominant sand fly species collected inside houses in Kfar Adumim, an Israeli village in the Judean Desert that is a focus of cutaneous leishmaniasis, was Phlebotomus papatasi, which was also caught attempting to bite humans. Phlebotomus sergenti, which is rarely seen inside houses, constituted the predominant sand fly species in caves near the village. Leishmania isolates from Ph. sergenti and humans typed as Leishmania tropica. Sand fly and human isolates produced similar small nodular cutaneous lesions in hamsters. Isolates produced excreted factor (EF) of subserotypes A9 or A9B2, characteristic of L. tropica and reacted with L. tropica-specific monoclonal antibodies. Isoenzyme analysis consigned the strains to the L. tropica zymodemes MON-137 and MON-275. Molecular genetic analyses confirmed the strains were L. tropica and intraspecific microheterogeneity was observed. Genomic fingerprinting using a mini-satellite probe separated the L. tropica strains into two clusters that were not entirely congruent with geographic distribution. These results support the heterogeneous nature of L. tropica and incriminate Ph. sergenti as its vector in this Judean Desert focus.
UR - http://www.scopus.com/inward/record.url?scp=11144358323&partnerID=8YFLogxK
U2 - 10.4269/ajtmh.2004.70.364
DO - 10.4269/ajtmh.2004.70.364
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C2 - 15100448
AN - SCOPUS:11144358323
SN - 0002-9637
VL - 70
SP - 364
EP - 372
JO - American Journal of Tropical Medicine and Hygiene
JF - American Journal of Tropical Medicine and Hygiene
IS - 4
ER -