Multiple controls on the intracellular trapping of uridine

W. D. Stein*, A. Klein

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Uridine uptake by mouse or hamster cells grown in conditions which support good growth is very sensitive to inhibition by cyanide and azide, at concentrations which only slightly reduce overall cellular ATP levels. Iodoacetate, when present alone, reduces uridine uptake only insofar as it reduces cellular ATP levels. At concentrations which by themselves do not affect uridine uptake, iodoacetate greatly reduces the sensitivity of uridine uptake to cyanide or azide. The effect of cyanide is on intracellular trapping of uridine and not on its transport into the cell. The specific effect of cyanide is confined to uridine and not found for the uptake of adenine, thymidine or 2-deoxyglucose. The effect is of rapid onset (within 2 min) and is rapidly reversible (also within 2 min). Phosphorylation of uridine in homogenised cells or in Triton X-100-permeabilised cells is unaffected by cyanide. The data are interpreted in terms of a model in which intracellular trapping of uridine is subject to multiple controls, including one regulated by some factor requiring intact functioning of the mitochondrion. These multiple control systems interact synergistically to affect trapping of uridine by the intact cell.

Original languageEnglish
Pages (from-to)94-101
Number of pages8
JournalBiochimica et Biophysica Acta - Molecular Cell Research
Volume762
Issue number1
DOIs
StatePublished - 16 Feb 1983

Keywords

  • (Fibroblast)
  • Uridine kinase
  • Uridine trapping

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