Multiple sites for double-strand breaks in whole meiotic chromosomes of Saccharomyces cerevisiae

Drora Zenvirth, Tamar Arbel, Amir Sherman, Martin Goldway, Shoshana Klein, Giora Simchen*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

128 Scopus citations

Abstract

We present a scheme for locating double-strand breaks (DSBs) in meiotic chromosomes of Saccharomyces cerevisiae, based on the separation of large DNA molecules by pulsed field gel electrophoresis. Using a rad50S mutant, in which DSBs are not processed, we show that DSBs are widely induced in S.cerevisiae chromosomes during meiosis. Some of the DSBs accumulate at certain preferred sites. We present general profiles of DSBs in chromosomes III, V, VI and VII. A map of the 12 preferred sites on chromosome III is presented. At least some of these sites correlate with known 'hot spots' for meiotic recombination. The data are discussed in view of current models of meiotic recombination and chromosome segregation.

Original languageEnglish
Pages (from-to)3441-3447
Number of pages7
JournalEMBO Journal
Volume11
Issue number9
StatePublished - Sep 1992

Keywords

  • Chromosomes
  • Double-strand breaks
  • Meiosis
  • Recombination
  • Saccharomyces cerevisiae

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