Multiplexing DNA methylation markers to detect circulating cell-free DNA derived from human pancreatic β cells

Daniel Neiman, David Gillis, Sheina Piyanzin, Daniel Cohen, Ori Fridlich, Joshua Moss, Aviad Zick, Tal Oron, Frida Sundberg, Gun Forsander, Oskar Skog, Olle Korsgren, Floris Levy-Khademi, Dan Arbel, Saar Hashavya, A. M. James Shapiro, Cate Speake, Carla Greenbaum, Jennifer Hosford, Amanda PosgaiMark A. Atkinson, Benjamin Glaser, Desmond A. Schatz*, Ruth Shemer*, Yuval Dor*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

31 Scopus citations

Abstract

It has been proposed that unmethylated insulin promoter fragments in plasma derive exclusively from β cells, reflect their recent demise, and can be used to assess β cell damage in type 1 diabetes. Herein we describe an ultrasensitive assay for detection of a β cell–specific DNA methylation signature, by simultaneous assessment of 6 DNA methylation markers, that identifies β cell DNA in mixtures containing as little as 0.03% β cell DNA (less than 1 β cell genome equivalent). Based on this assay, plasma from nondiabetic individuals (N = 218, aged 4–78 years) contained on average only 1 β cell genome equivalent/mL. As expected, cell-free DNA (cfDNA) from β cells was significantly elevated in islet transplant recipients shortly after transplantation. We also detected β cell cfDNA in a patient with KATP congenital hyperinsulinism, in which substantial β cell turnover is thought to occur. Strikingly, in contrast to previous reports, we observed no elevation of β cell–derived cfDNA in autoantibody-positive subjects at risk for type 1 diabetes (N = 32), individuals with recent-onset type 1 diabetes (<4 months, N = 92), or those with long-standing disease (>4 months, N = 38). We discuss the utility of sensitive β cell cfDNA analysis and potential explanations for the lack of a β cell cfDNA signal in type 1 diabetes.

Original languageAmerican English
Article numbere136579
JournalJCI insight
Volume5
Issue number14
DOIs
StatePublished - 23 Jun 2020

Bibliographical note

Publisher Copyright:
Copyright: © 2020, American Society for Clinical Investigation.

Fingerprint

Dive into the research topics of 'Multiplexing DNA methylation markers to detect circulating cell-free DNA derived from human pancreatic β cells'. Together they form a unique fingerprint.

Cite this