Multispectral labeling technique to map many neighboring axonal projections in the same tissue

Shlomo Tsuriel, Sagi Gudes, Ryan W. Draft, Alexander M. Binshtok*, Jeff W. Lichtman

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

22 Scopus citations


We describe a method to map the location of axonal arbors of many individual neurons simultaneously via the spectral properties of retrogradely transported dye-labeled vesicles. We inject overlapping regions of an axon target area with three or more different colored retrograde tracers. On the basis of the combinations and intensities of the colors in the individual vesicles transported to neuronal somata, we calculate the projection sites of each neuron's axon. This neuronal positioning system (NPS) enables mapping of many axons in a simple automated way. In our experiments, NPS combined with spectral (Brainbow) labeling of the input to autonomic ganglion cells showed that the locations of ganglion cell projections to a mouse salivary gland related to the identities of their preganglionic axonal innervation. NPS could also delineate projections of many axons simultaneously in the mouse central nervous system.

Original languageAmerican English
Pages (from-to)547-552
Number of pages6
JournalNature Methods
Issue number6
StatePublished - 28 May 2015

Bibliographical note

Publisher Copyright:
© 2015 Nature America, Inc. All rights reserved.


Dive into the research topics of 'Multispectral labeling technique to map many neighboring axonal projections in the same tissue'. Together they form a unique fingerprint.

Cite this