Multistep mechanism of probiotic bacterium, the effect on innate immune system

Einat Amit-Romach, Zehava Uni, Ram Reifen*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

41 Scopus citations


The etiology and pathogenesis of inflammatory bowel disease are still not fully understood. However, evidence from both animal models and clinical observations suggests luminal bacteria as the most probable inducer of this disease. The intestinal bacterial microbiota may be modified by dietary addition of viable probiotic bacteria, thereby constituting an alternative approach to disease prevention and treatment. The aim of this study was to evaluate and compare the effects of two probiotic regiments; Lactobacillus GG and a mixture of Streptococcus thermophilus, Lactobacillus acidophilus, and Bifidobacterium lactis (YO-MIX™ Y 109 FRO 1000) in both normal and trinitrobenzenesulfonic acid colitis-induced rats. Colon morphology and damage were evaluated histologically; colonic tissues were used for mRNA analysis, using real-time PCR. Administration of both probiotics reduced the expression of proinflammatory cytokines tumor necrosis factor-a and IL-6 and increased the expression of mucin 2 in compared with colitis group and reduced the inflammatory response. These results provide additional support for the positive effect of probiotics in the gut and may shed light on the mechanism by which probiotic bacteria exert their action in an animal model.

Original languageAmerican English
Pages (from-to)277-284
Number of pages8
JournalMolecular Nutrition and Food Research
Issue number2
StatePublished - Feb 2010


  • Experimental colitis
  • Gene expression
  • Inflammatory bowel disease
  • Microbiota
  • Probiotics


Dive into the research topics of 'Multistep mechanism of probiotic bacterium, the effect on innate immune system'. Together they form a unique fingerprint.

Cite this