Muricauda antarctica sp. nov., a marine member of the Flavobacteriaceae isolated from Antarctic seawater

Yue Hong Wu, Pei Song Yu, Ya Dong Zhou, Lin Xu, Chun Sheng Wang, Min Wu, Aharon Oren, Xue Wei Xu*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

28 Scopus citations

Abstract

A Gram-stain-negative, rod-shaped bacterium with appendages, designated Ar-22T, was isolated from a seawater sample collected from the western part of Prydz Bay, near Cape Darnley, Antarctica. Strain Ar-22T grew optimally at 35 °C, at pH 7.5 and in the presence of 1-3% (w/v) NaCl. The isolate was positive for casein, gelatin and Tween 20 decomposition and negative for H2S production and indole formation. Chemotaxonomic analysis showed that MK-6 was the major isoprenoid quinone and phosphatidylethanolamine was the major polar lipid. The major fatty acids were iso-C17:0 3-OH, iso-C15:1 G, iso-C15:0 and C16:1ω7c/iso-C15:0 2OH. The genomic DNA G + C content was 44.8 mol%. Comparative 16S rRNA gene sequence analysis revealed that strain Ar-22T is closely related to members of the genus Muricauda, sharing 94.2-97.3% sequence similarity with the type strains of species of the genus Muricauda and being most closely related to the Muricauda aquimarina. Phylogenetic analysis based on the 16S rRNA gene sequence comparison confirmed that strain Ar-22T formed a deep lineage with Muricauda flavescens. Sequence similarity between strain Ar-22T and Muricauda ruestringensis DSM 13258T, the type species of the genus Muricauda, was 96.9%. Strain Ar-22T exhibited mean DNA-DNA relatedness values of 40.1 %, 49.4% and 25.7 % to M. aquimarina JCM 11811T, M. flavescens JCM 11812T and Muricauda lutimaris KCTC 22173T, respectively. On the basis of phenotypic and genotypic data, strain Ar-22T represents a novel species of the genus Muricauda, for which the name Muricauda antarctica sp. nov. (type strain Ar-22T=CGMCC 1.12174T=JCM 18450T) is proposed.

Original languageAmerican English
Pages (from-to)3451-3456
Number of pages6
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume63
Issue numberPART9
DOIs
StatePublished - 2013

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