Murine and human mast cell express acetylcholinesterase

Hovav Nechushtan; Hermona Soreq; Valentina Kuperstein; Sagi Tshori; Ehud Razin

doi:10.1016/0014-5793(95)01466-7

Murine and human mast cell express acetylcholinesterase

Hovav Nechushtan, Hermona Soreq, Valentina Kuperstein, Sagi Tshori, Ehud Razin^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

Expression of catalytically active protein was detected in a murine mast cell line. The primary type of AChE mRNA produced by these cells was found to be the brain and muscle type by PCR amplification of alternative exons from the 3' of mast cells AChE cDNA. AChE was further found to be expressed in the HMC-1 the human mast cell precursor line. Furthermore, utilizing the single cell RT-PCR method we detected AChE mRNA expression in FcεRI-positive single cells derived from human colonic mucosal biopsies. Our findings predict the involvement of mast cell AChE in neuronal-mast cell interactions.

Original language	English
Pages (from-to)	1-6
Number of pages	6
Journal	FEBS Letters
Volume	379
Issue number	1
DOIs	https://doi.org/10.1016/0014-5793(95)01466-7
State	Published - 22 Jan 1996

Keywords

AChE
Alternative splicing
Human precursor cell
Mast cell
Single-cell RT-PCR

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10.1016/0014-5793(95)01466-7

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abstract = "Expression of catalytically active protein was detected in a murine mast cell line. The primary type of AChE mRNA produced by these cells was found to be the brain and muscle type by PCR amplification of alternative exons from the 3' of mast cells AChE cDNA. AChE was further found to be expressed in the HMC-1 the human mast cell precursor line. Furthermore, utilizing the single cell RT-PCR method we detected AChE mRNA expression in FcεRI-positive single cells derived from human colonic mucosal biopsies. Our findings predict the involvement of mast cell AChE in neuronal-mast cell interactions.",

keywords = "AChE, Alternative splicing, Human precursor cell, Mast cell, Single-cell RT-PCR",

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AU - Soreq, Hermona

AU - Kuperstein, Valentina

AU - Tshori, Sagi

AU - Razin, Ehud

PY - 1996/1/22

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N2 - Expression of catalytically active protein was detected in a murine mast cell line. The primary type of AChE mRNA produced by these cells was found to be the brain and muscle type by PCR amplification of alternative exons from the 3' of mast cells AChE cDNA. AChE was further found to be expressed in the HMC-1 the human mast cell precursor line. Furthermore, utilizing the single cell RT-PCR method we detected AChE mRNA expression in FcεRI-positive single cells derived from human colonic mucosal biopsies. Our findings predict the involvement of mast cell AChE in neuronal-mast cell interactions.

AB - Expression of catalytically active protein was detected in a murine mast cell line. The primary type of AChE mRNA produced by these cells was found to be the brain and muscle type by PCR amplification of alternative exons from the 3' of mast cells AChE cDNA. AChE was further found to be expressed in the HMC-1 the human mast cell precursor line. Furthermore, utilizing the single cell RT-PCR method we detected AChE mRNA expression in FcεRI-positive single cells derived from human colonic mucosal biopsies. Our findings predict the involvement of mast cell AChE in neuronal-mast cell interactions.

KW - AChE

KW - Alternative splicing

KW - Human precursor cell

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KW - Single-cell RT-PCR

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Murine and human mast cell express acetylcholinesterase

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