Abstract
Natural killer (NK) cells are important for their ability to recognize and lyse tumor cells and virus infected cells. NK cells express triggering receptors that are specific for non-MHC ligands. This article describes the 35S release cytotoxic assay, which measures the ability of NK cells derived from spleen cells taken from polyIC-treated mice to lyse B-cell leukemia (BCL1) cells. BCL1 cells express ligands for NKp46 on the cell surface membrane and they are sensitive to allogeneic but not syngeneic IL-2 activated natural killer cells.
Original language | American English |
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Pages (from-to) | 1137-1141 |
Number of pages | 5 |
Journal | Bone Marrow Transplantation |
Volume | 33 |
Issue number | 11 |
DOIs | |
State | Published - 1 Jun 2004 |
Bibliographical note
Funding Information:We thank the Danny Cunniff Leukemia Research Laboratory; the Gabrielle Rich Leukemia Research Foundation; the Cancer Treatment Research Foundation; the Novotny Trust and the Fig Tree Foundation for their continuous support of our ongoing basic and clinical research.
Keywords
- B-cell leukemia (BCL)
- Immunity
- Lymphokine activated killer cells (LAK cells)
- Murine