Murine Soft Tissue Infection Model to Study Group A Streptococcus (GAS) Pathogenesis in Necrotizing Fasciitis

Miriam Ravins; Poornima Ambalavanan; Debabrata Biswas; Rachel Ying Min Tan; Kimberly Xuan Zhen Lim; Yael Kaufman; Aparna Anand; Abhinay Sharma; Emanuel Hanski

doi:10.1007/978-1-0716-1971-1_16

Murine Soft Tissue Infection Model to Study Group A Streptococcus (GAS) Pathogenesis in Necrotizing Fasciitis

Miriam Ravins
, Poornima Ambalavanan
, Debabrata Biswas
, Rachel Ying Min Tan
, Kimberly Xuan Zhen Lim
, Yael Kaufman
, Aparna Anand
, Abhinay Sharma
, Emanuel Hanski^*

^*Corresponding author for this work

Institute for Medical Research Israel-Canada (IMRIC)

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

5 Scopus citations

Abstract

Group A streptococcus (GAS) necrotizing fasciitis (NF) causes high morbidity and mortality despite prompt intravenous administration of antibiotics, surgical soft-tissue debridement, and supportive treatment in the intensive care unit. Since there is no effective vaccine against GAS infections, a comprehensive understanding of NF pathogenesis is required to design more efficient treatments. To increase our understanding of NF pathogenesis, we need a reliable animal model that mirrors, at least in part, the infectious process in humans. This chapter describes a reliable murine model of human NF that mimics the histopathology observed in humans, namely the destruction of soft tissue, a paucity of infiltrating neutrophils, and the presence of many gram-positive cocci at the center of the infection.

Original language	English
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	185-200
Number of pages	16
DOIs	https://doi.org/10.1007/978-1-0716-1971-1_16
State	Published - 2022

Publication series

Name	Methods in Molecular Biology
Volume	2427
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Bibliographical note

Publisher Copyright:
© 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Keywords

Animal model
Fascia
Gram-positive cocci
Group A streptococcus
Immunohistopathology
Necrosis
Necrotizing fasciitis
Neutrophils

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10.1007/978-1-0716-1971-1_16

Cite this

Ravins, M., Ambalavanan, P., Biswas, D., Tan, R. Y. M., Lim, K. X. Z., Kaufman, Y., Anand, A., Sharma, A., & Hanski, E. (2022). Murine Soft Tissue Infection Model to Study Group A Streptococcus (GAS) Pathogenesis in Necrotizing Fasciitis. In Methods in Molecular Biology (pp. 185-200). (Methods in Molecular Biology; Vol. 2427). Humana Press Inc.. https://doi.org/10.1007/978-1-0716-1971-1_16

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abstract = "Group A streptococcus (GAS) necrotizing fasciitis (NF) causes high morbidity and mortality despite prompt intravenous administration of antibiotics, surgical soft-tissue debridement, and supportive treatment in the intensive care unit. Since there is no effective vaccine against GAS infections, a comprehensive understanding of NF pathogenesis is required to design more efficient treatments. To increase our understanding of NF pathogenesis, we need a reliable animal model that mirrors, at least in part, the infectious process in humans. This chapter describes a reliable murine model of human NF that mimics the histopathology observed in humans, namely the destruction of soft tissue, a paucity of infiltrating neutrophils, and the presence of many gram-positive cocci at the center of the infection.",

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author = "Miriam Ravins and Poornima Ambalavanan and Debabrata Biswas and Tan, \{Rachel Ying Min\} and Lim, \{Kimberly Xuan Zhen\} and Yael Kaufman and Aparna Anand and Abhinay Sharma and Emanuel Hanski",

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year = "2022",

doi = "10.1007/978-1-0716-1971-1\_16",

language = "אנגלית",

series = "Methods in Molecular Biology",

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Murine Soft Tissue Infection Model to Study Group A Streptococcus (GAS) Pathogenesis in Necrotizing Fasciitis. / Ravins, Miriam; Ambalavanan, Poornima; Biswas, Debabrata et al.
Methods in Molecular Biology. Humana Press Inc., 2022. p. 185-200 (Methods in Molecular Biology; Vol. 2427).

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

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T1 - Murine Soft Tissue Infection Model to Study Group A Streptococcus (GAS) Pathogenesis in Necrotizing Fasciitis

AU - Ravins, Miriam

AU - Ambalavanan, Poornima

AU - Biswas, Debabrata

AU - Tan, Rachel Ying Min

AU - Lim, Kimberly Xuan Zhen

AU - Kaufman, Yael

AU - Anand, Aparna

AU - Sharma, Abhinay

AU - Hanski, Emanuel

PY - 2022

Y1 - 2022

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AB - Group A streptococcus (GAS) necrotizing fasciitis (NF) causes high morbidity and mortality despite prompt intravenous administration of antibiotics, surgical soft-tissue debridement, and supportive treatment in the intensive care unit. Since there is no effective vaccine against GAS infections, a comprehensive understanding of NF pathogenesis is required to design more efficient treatments. To increase our understanding of NF pathogenesis, we need a reliable animal model that mirrors, at least in part, the infectious process in humans. This chapter describes a reliable murine model of human NF that mimics the histopathology observed in humans, namely the destruction of soft tissue, a paucity of infiltrating neutrophils, and the presence of many gram-positive cocci at the center of the infection.

KW - Animal model

KW - Fascia

KW - Gram-positive cocci

KW - Group A streptococcus

KW - Immunohistopathology

KW - Necrosis

KW - Necrotizing fasciitis

KW - Neutrophils

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T3 - Methods in Molecular Biology

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PB - Humana Press Inc.

ER -

Murine Soft Tissue Infection Model to Study Group A Streptococcus (GAS) Pathogenesis in Necrotizing Fasciitis

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Keywords

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