Muscle Contraction Is Necessary to Maintain Joint Progenitor Cell Fate

Joy Kahn; Yulia Shwartz; Einat Blitz; Sharon Krief; Amnon Sharir; Dario A. Breitel; Revital Rattenbach; Frederic Relaix; Pascal Maire; Ryan B. Rountree; David M. Kingsley; Elazar Zelzer

doi:10.1016/j.devcel.2009.04.013

Muscle Contraction Is Necessary to Maintain Joint Progenitor Cell Fate

Joy Kahn, Yulia Shwartz, Einat Blitz, Sharon Krief, Amnon Sharir, Dario A. Breitel, Revital Rattenbach, Frederic Relaix, Pascal Maire, Ryan B. Rountree, David M. Kingsley, Elazar Zelzer^*

^*Corresponding author for this work

Institute of Biomedical Research at the Faculty of Dental Medicine

Research output: Contribution to journal › Article › peer-review

193 Scopus citations

Abstract

During embryogenesis, organ development is dependent upon maintaining appropriate progenitor cell commitment. Synovial joints develop from a pool of progenitor cells that differentiate into various cell types constituting the mature joint. The involvement of the musculature in joint formation has long been recognized. However, the mechanism by which the musculature regulates joint formation has remained elusive. In this study, we demonstrate, utilizing various murine models devoid of limb musculature or its contraction, that the contracting musculature is fundamental in maintaining joint progenitors committed to their fate, a requirement for correct joint cavitation and morphogenesis. Furthermore, contraction-dependent activation of β-catenin, a key modulator of joint formation, provides a molecular mechanism for this regulation. In conclusion, our findings provide the missing link between progenitor cell fate determination and embryonic movement, two processes shown to be essential for correct organogenesis.

Original language	American English
Pages (from-to)	734-743
Number of pages	10
Journal	Developmental Cell
Volume	16
Issue number	5
DOIs	https://doi.org/10.1016/j.devcel.2009.04.013
State	Published - 19 May 2009

Bibliographical note

Funding Information:
We are grateful to George Kern, Innsbruck, Austria, for the mdg mice and to C. Hartmann, Dr. Y. Yang, M. Pacifici, R. Boot-Handford, and A. Vortkamp for RNA probes. The authors wish to thank C.J. Tabin, J.G. Hall, and R. Schweitzer for suggestions and beneficial discussions, D.J. Glotzer, C. Hartmann, and W. McLean for their helpful reviews of the manuscript, and N. Konstantin for expert editorial assistance. Special thanks to all members of the Zelzer laboratory for advice and suggestions. This work was supported by The Clore Center for Biological Physics, The Leo and Julia Forchheimer Center for Molecular Genetics, The Stanley Chais New Scientist Fund, The Kirk Center for Childhood Cancer and Immunological Disorders, and The David and Fela Shapell Family Center for Genetic Disorders Research. E.Z. is the incumbent of the Martha S. Sagon Career Development Chair.

Keywords

DEVBIO

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10.1016/j.devcel.2009.04.013

Cite this

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title = "Muscle Contraction Is Necessary to Maintain Joint Progenitor Cell Fate",

abstract = "During embryogenesis, organ development is dependent upon maintaining appropriate progenitor cell commitment. Synovial joints develop from a pool of progenitor cells that differentiate into various cell types constituting the mature joint. The involvement of the musculature in joint formation has long been recognized. However, the mechanism by which the musculature regulates joint formation has remained elusive. In this study, we demonstrate, utilizing various murine models devoid of limb musculature or its contraction, that the contracting musculature is fundamental in maintaining joint progenitors committed to their fate, a requirement for correct joint cavitation and morphogenesis. Furthermore, contraction-dependent activation of β-catenin, a key modulator of joint formation, provides a molecular mechanism for this regulation. In conclusion, our findings provide the missing link between progenitor cell fate determination and embryonic movement, two processes shown to be essential for correct organogenesis.",

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author = "Joy Kahn and Yulia Shwartz and Einat Blitz and Sharon Krief and Amnon Sharir and Breitel, {Dario A.} and Revital Rattenbach and Frederic Relaix and Pascal Maire and Rountree, {Ryan B.} and Kingsley, {David M.} and Elazar Zelzer",

note = "Funding Information: We are grateful to George Kern, Innsbruck, Austria, for the mdg mice and to C. Hartmann, Dr. Y. Yang, M. Pacifici, R. Boot-Handford, and A. Vortkamp for RNA probes. The authors wish to thank C.J. Tabin, J.G. Hall, and R. Schweitzer for suggestions and beneficial discussions, D.J. Glotzer, C. Hartmann, and W. McLean for their helpful reviews of the manuscript, and N. Konstantin for expert editorial assistance. Special thanks to all members of the Zelzer laboratory for advice and suggestions. This work was supported by The Clore Center for Biological Physics, The Leo and Julia Forchheimer Center for Molecular Genetics, The Stanley Chais New Scientist Fund, The Kirk Center for Childhood Cancer and Immunological Disorders, and The David and Fela Shapell Family Center for Genetic Disorders Research. E.Z. is the incumbent of the Martha S. Sagon Career Development Chair. ",

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AU - Kahn, Joy

AU - Shwartz, Yulia

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AU - Krief, Sharon

AU - Sharir, Amnon

AU - Breitel, Dario A.

AU - Rattenbach, Revital

AU - Relaix, Frederic

AU - Maire, Pascal

AU - Rountree, Ryan B.

AU - Kingsley, David M.

AU - Zelzer, Elazar

N1 - Funding Information: We are grateful to George Kern, Innsbruck, Austria, for the mdg mice and to C. Hartmann, Dr. Y. Yang, M. Pacifici, R. Boot-Handford, and A. Vortkamp for RNA probes. The authors wish to thank C.J. Tabin, J.G. Hall, and R. Schweitzer for suggestions and beneficial discussions, D.J. Glotzer, C. Hartmann, and W. McLean for their helpful reviews of the manuscript, and N. Konstantin for expert editorial assistance. Special thanks to all members of the Zelzer laboratory for advice and suggestions. This work was supported by The Clore Center for Biological Physics, The Leo and Julia Forchheimer Center for Molecular Genetics, The Stanley Chais New Scientist Fund, The Kirk Center for Childhood Cancer and Immunological Disorders, and The David and Fela Shapell Family Center for Genetic Disorders Research. E.Z. is the incumbent of the Martha S. Sagon Career Development Chair.

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N2 - During embryogenesis, organ development is dependent upon maintaining appropriate progenitor cell commitment. Synovial joints develop from a pool of progenitor cells that differentiate into various cell types constituting the mature joint. The involvement of the musculature in joint formation has long been recognized. However, the mechanism by which the musculature regulates joint formation has remained elusive. In this study, we demonstrate, utilizing various murine models devoid of limb musculature or its contraction, that the contracting musculature is fundamental in maintaining joint progenitors committed to their fate, a requirement for correct joint cavitation and morphogenesis. Furthermore, contraction-dependent activation of β-catenin, a key modulator of joint formation, provides a molecular mechanism for this regulation. In conclusion, our findings provide the missing link between progenitor cell fate determination and embryonic movement, two processes shown to be essential for correct organogenesis.

AB - During embryogenesis, organ development is dependent upon maintaining appropriate progenitor cell commitment. Synovial joints develop from a pool of progenitor cells that differentiate into various cell types constituting the mature joint. The involvement of the musculature in joint formation has long been recognized. However, the mechanism by which the musculature regulates joint formation has remained elusive. In this study, we demonstrate, utilizing various murine models devoid of limb musculature or its contraction, that the contracting musculature is fundamental in maintaining joint progenitors committed to their fate, a requirement for correct joint cavitation and morphogenesis. Furthermore, contraction-dependent activation of β-catenin, a key modulator of joint formation, provides a molecular mechanism for this regulation. In conclusion, our findings provide the missing link between progenitor cell fate determination and embryonic movement, two processes shown to be essential for correct organogenesis.

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ER -

Muscle Contraction Is Necessary to Maintain Joint Progenitor Cell Fate

Abstract

Bibliographical note

Keywords

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