Muscle-specific activation of a methylated chimeric actin gene

Joel Yisraeli*, Robert S. Adelstein, Danielle Melloul, Uri Nudel, David Yaffe, Howard Cedar

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

92 Scopus citations

Abstract

To understand how DNA methylation affects tissue-specific activation of genes, we have transfected in vitro methylated α-actin (skeletal) constructs into fibroblasts, which do not produce endogenous α-actin, and into a myogenic line, which is inducible for α-actin expression. Although methylation significantly inhibits the expression of these constructs in fibroblasts, it does not in myoblasts. The methylation pattern of the introduced methylated genes reveals specific demethylations in the transfected molecules in myoblasts but not in fibroblasts, and it precisely mimics the methylation pattern found in myoblasts in vivo.

Original languageAmerican English
Pages (from-to)409-416
Number of pages8
JournalCell
Volume46
Issue number3
DOIs
StatePublished - 1 Aug 1986

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