Muscle-specific activation of a methylated chimeric actin gene

To understand how DNA methylation affects tissue-specific activation of genes, we have transfected in vitro methylated α-actin (skeletal) constructs into fibroblasts, which do not produce endogenous α-actin, and into a myogenic line, which is inducible for α-actin expression. Although methylation significantly inhibits the expression of these constructs in fibroblasts, it does not in myoblasts. The methylation pattern of the introduced methylated genes reveals specific demethylations in the transfected molecules in myoblasts but not in fibroblasts, and it precisely mimics the methylation pattern found in myoblasts in vivo.