TY - JOUR
T1 - Mutations affecting substrate specificity of the Bacillus subtilis multidrug transporter Bmr
AU - Klyachko, Katya A.
AU - Schuldiner, Shimon
AU - Neyfakh, Alexander A.
PY - 1997
Y1 - 1997
N2 - The Bacillus subtilis multidrug transporter Bmr, a member of the major facilitator superfamily of transporters, causes the efflux of a number of structurally unrelated toxic compounds from cells. We have shown previously that the activity of Bmr can he inhibited by the plant alkaloid reserpine. Here we demonstrate that various substitutions of residues Phe143 and Phe306 of Bmr not only reduce its sensitivity to reserpine inhibition but also significantly change its substrate specificity. Cross-resistance profiles of bacteria expressing mutant forms of the transporter differ from each other and from the cross-resistance profile of cells expressing wild- type Bmr. This result strongly suggests that Bmr interacts with its transported drugs directly, with residues Phe143 and Phe306 likely to be involved in substrate recognition.
AB - The Bacillus subtilis multidrug transporter Bmr, a member of the major facilitator superfamily of transporters, causes the efflux of a number of structurally unrelated toxic compounds from cells. We have shown previously that the activity of Bmr can he inhibited by the plant alkaloid reserpine. Here we demonstrate that various substitutions of residues Phe143 and Phe306 of Bmr not only reduce its sensitivity to reserpine inhibition but also significantly change its substrate specificity. Cross-resistance profiles of bacteria expressing mutant forms of the transporter differ from each other and from the cross-resistance profile of cells expressing wild- type Bmr. This result strongly suggests that Bmr interacts with its transported drugs directly, with residues Phe143 and Phe306 likely to be involved in substrate recognition.
UR - http://www.scopus.com/inward/record.url?scp=0030949566&partnerID=8YFLogxK
U2 - 10.1128/jb.179.7.2189-2193.1997
DO - 10.1128/jb.179.7.2189-2193.1997
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C2 - 9079903
AN - SCOPUS:0030949566
SN - 0021-9193
VL - 179
SP - 2189
EP - 2193
JO - Journal of Bacteriology
JF - Journal of Bacteriology
IS - 7
ER -