TY - JOUR
T1 - Mycobacteria possess a surprisingly small number of ribosomal RNA genes in relation to the size of their genome
AU - Bercovier, Herve
AU - Kafri, Orna
AU - Sela, Shlomo
PY - 1986/5/14
Y1 - 1986/5/14
N2 - DNAs from Mycobacterium tuberculosis, M. intracellulare, M. phlei and M. smegmatis were digested by restriction enzymes and hybridized with three probes consisting of the 5′ (16S rRNA), the middle (16S and 23S rRNA), and the 3′ (23S and 5S rRNA) portions of the Escherichia coli rrnB operon. The resulting hybridization patterns indicate that slow-growing Mycobacteria species (i.e., M. tuberculosis and M. intracellulare), with genome size 3.13 - 4.29 × 109 daltons, appear to possess only one rRNA operon, whereas fast-growing species (i.e., M. phlei and M. smegmatis, with genome size 4.30 - 5.20 × 109 daltons, appear to possess two rRNA operons.
AB - DNAs from Mycobacterium tuberculosis, M. intracellulare, M. phlei and M. smegmatis were digested by restriction enzymes and hybridized with three probes consisting of the 5′ (16S rRNA), the middle (16S and 23S rRNA), and the 3′ (23S and 5S rRNA) portions of the Escherichia coli rrnB operon. The resulting hybridization patterns indicate that slow-growing Mycobacteria species (i.e., M. tuberculosis and M. intracellulare), with genome size 3.13 - 4.29 × 109 daltons, appear to possess only one rRNA operon, whereas fast-growing species (i.e., M. phlei and M. smegmatis, with genome size 4.30 - 5.20 × 109 daltons, appear to possess two rRNA operons.
UR - http://www.scopus.com/inward/record.url?scp=0022531262&partnerID=8YFLogxK
U2 - 10.1016/0006-291X(86)90452-3
DO - 10.1016/0006-291X(86)90452-3
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C2 - 3013168
AN - SCOPUS:0022531262
SN - 0006-291X
VL - 136
SP - 1136
EP - 1141
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -
