Abstract
Mycoplasmas have been incriminated in setting the stage for HIV infection and full-blown AIDS. We tested the possible involvement of mycoplasmas in activation of HIV. Two cell lines, 293 fibroblasts and Jurkat CD4+ T-cells, transfected with plasmids harboring a transcription fusion construct between HIV-long terminal repeat (HIV-LTR) and either luc or cat genes, were infected with several mycoplasmas (M. fermentons; M. penetrons M. pirum and Ureaplasma urealyticum) and the reporter gene expression was monitored. The data presented here suggest that mycoplasmas, and specifically their membranes, play a role in the activation of HIV-LTR mediated transcription.
Original language | English |
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Pages (from-to) | 63-68 |
Number of pages | 6 |
Journal | FEMS Microbiology Letters |
Volume | 128 |
Issue number | 1 |
DOIs | |
State | Published - 15 Apr 1995 |
Keywords
- HIV
- HIV-long terminal repeat
- Light detection in situ
- Luciferase
- Luciferin
- Mycoplasma
- Transactivation