Mycoplasmas regulate HIV-LTR-dependent gene expression

Ran Nir-paz, Shoshana Israel, Alik Honigman, Itzhak Kahane*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

Mycoplasmas have been incriminated in setting the stage for HIV infection and full-blown AIDS. We tested the possible involvement of mycoplasmas in activation of HIV. Two cell lines, 293 fibroblasts and Jurkat CD4+ T-cells, transfected with plasmids harboring a transcription fusion construct between HIV-long terminal repeat (HIV-LTR) and either luc or cat genes, were infected with several mycoplasmas (M. fermentons; M. penetrons M. pirum and Ureaplasma urealyticum) and the reporter gene expression was monitored. The data presented here suggest that mycoplasmas, and specifically their membranes, play a role in the activation of HIV-LTR mediated transcription.

Original languageEnglish
Pages (from-to)63-68
Number of pages6
JournalFEMS Microbiology Letters
Volume128
Issue number1
DOIs
StatePublished - 15 Apr 1995

Keywords

  • HIV
  • HIV-long terminal repeat
  • Light detection in situ
  • Luciferase
  • Luciferin
  • Mycoplasma
  • Transactivation

Fingerprint

Dive into the research topics of 'Mycoplasmas regulate HIV-LTR-dependent gene expression'. Together they form a unique fingerprint.

Cite this