Mycoplasmas regulate HIV-LTR-dependent gene expression

Ran Nir-paz; Shoshana Israel; Alik Honigman; Itzhak Kahane

doi:10.1016/0378-1097(95)00084-I

Mycoplasmas regulate HIV-LTR-dependent gene expression

Ran Nir-paz
, Shoshana Israel
, Alik Honigman
, Itzhak Kahane^*

^*Corresponding author for this work

Institute for Medical Research Israel-Canada (IMRIC)

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

Mycoplasmas have been incriminated in setting the stage for HIV infection and full-blown AIDS. We tested the possible involvement of mycoplasmas in activation of HIV. Two cell lines, 293 fibroblasts and Jurkat CD4+ T-cells, transfected with plasmids harboring a transcription fusion construct between HIV-long terminal repeat (HIV-LTR) and either luc or cat genes, were infected with several mycoplasmas (M. fermentons; M. penetrons M. pirum and Ureaplasma urealyticum) and the reporter gene expression was monitored. The data presented here suggest that mycoplasmas, and specifically their membranes, play a role in the activation of HIV-LTR mediated transcription.

Original language	English
Pages (from-to)	63-68
Number of pages	6
Journal	FEMS Microbiology Letters
Volume	128
Issue number	1
DOIs	https://doi.org/10.1016/0378-1097(95)00084-I
State	Published - 15 Apr 1995

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This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

HIV
HIV-long terminal repeat
Light detection in situ
Luciferase
Luciferin
Mycoplasma
Transactivation

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10.1016/0378-1097(95)00084-I

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abstract = "Mycoplasmas have been incriminated in setting the stage for HIV infection and full-blown AIDS. We tested the possible involvement of mycoplasmas in activation of HIV. Two cell lines, 293 fibroblasts and Jurkat CD4+ T-cells, transfected with plasmids harboring a transcription fusion construct between HIV-long terminal repeat (HIV-LTR) and either luc or cat genes, were infected with several mycoplasmas (M. fermentons; M. penetrons M. pirum and Ureaplasma urealyticum) and the reporter gene expression was monitored. The data presented here suggest that mycoplasmas, and specifically their membranes, play a role in the activation of HIV-LTR mediated transcription.",

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AU - Honigman, Alik

AU - Kahane, Itzhak

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AB - Mycoplasmas have been incriminated in setting the stage for HIV infection and full-blown AIDS. We tested the possible involvement of mycoplasmas in activation of HIV. Two cell lines, 293 fibroblasts and Jurkat CD4+ T-cells, transfected with plasmids harboring a transcription fusion construct between HIV-long terminal repeat (HIV-LTR) and either luc or cat genes, were infected with several mycoplasmas (M. fermentons; M. penetrons M. pirum and Ureaplasma urealyticum) and the reporter gene expression was monitored. The data presented here suggest that mycoplasmas, and specifically their membranes, play a role in the activation of HIV-LTR mediated transcription.

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KW - Luciferase

KW - Luciferin

KW - Mycoplasma

KW - Transactivation

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Mycoplasmas regulate HIV-LTR-dependent gene expression

Abstract

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Keywords

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