NAD/NADH-Sensitive quantum dots: Applications to probe nad-dependent enzymes and to sense the rdx explosive

Ronit Freeman; Itamar Willner

doi:10.1021/nl8030532

NAD/NADH-Sensitive quantum dots: Applications to probe nad-dependent enzymes and to sense the rdx explosive

Ronit Freeman, Itamar Willner^*

^*Corresponding author for this work

Institute of Chemistry

Research output: Contribution to journal › Article › peer-review

111 Scopus citations

Abstract

Phenyl boronic acid-tunctionalized CdSe/ZnS quantum dots (QDs) were synthesized. The modified particles bind nicotinamide adenine dinucleotide (NAD⁺) or 1,4-dihydronicotinamide adenine dinucleotide (NADH). The NAD⁺-functionalized QDs are effectively quenched by an electron transfer process, while the NADH-modified QDs are inefficiently quenched by the reduced cofactor. These properties enable the implementation of the QDs for the fluorescence analysis of ethanol in the presence of alcohol dehydrogenase. The NADH-functionalized QDs were used for the optical analysis of the 1,3,5-trinftrotriazine, RDX explosive, with a detection limit that corresponded to 1 × 10^-10 M. We demonstrate cooperative optical and catalytic functions of the core-shell components of the QDs in the analysis of RDX.

Original language	English
Pages (from-to)	322-326
Number of pages	5
Journal	Nano Letters
Volume	9
Issue number	1
DOIs	https://doi.org/10.1021/nl8030532
State	Published - Jan 2009

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abstract = "Phenyl boronic acid-tunctionalized CdSe/ZnS quantum dots (QDs) were synthesized. The modified particles bind nicotinamide adenine dinucleotide (NAD+) or 1,4-dihydronicotinamide adenine dinucleotide (NADH). The NAD+-functionalized QDs are effectively quenched by an electron transfer process, while the NADH-modified QDs are inefficiently quenched by the reduced cofactor. These properties enable the implementation of the QDs for the fluorescence analysis of ethanol in the presence of alcohol dehydrogenase. The NADH-functionalized QDs were used for the optical analysis of the 1,3,5-trinftrotriazine, RDX explosive, with a detection limit that corresponded to 1 × 10-10 M. We demonstrate cooperative optical and catalytic functions of the core-shell components of the QDs in the analysis of RDX.",

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N2 - Phenyl boronic acid-tunctionalized CdSe/ZnS quantum dots (QDs) were synthesized. The modified particles bind nicotinamide adenine dinucleotide (NAD+) or 1,4-dihydronicotinamide adenine dinucleotide (NADH). The NAD+-functionalized QDs are effectively quenched by an electron transfer process, while the NADH-modified QDs are inefficiently quenched by the reduced cofactor. These properties enable the implementation of the QDs for the fluorescence analysis of ethanol in the presence of alcohol dehydrogenase. The NADH-functionalized QDs were used for the optical analysis of the 1,3,5-trinftrotriazine, RDX explosive, with a detection limit that corresponded to 1 × 10-10 M. We demonstrate cooperative optical and catalytic functions of the core-shell components of the QDs in the analysis of RDX.

AB - Phenyl boronic acid-tunctionalized CdSe/ZnS quantum dots (QDs) were synthesized. The modified particles bind nicotinamide adenine dinucleotide (NAD+) or 1,4-dihydronicotinamide adenine dinucleotide (NADH). The NAD+-functionalized QDs are effectively quenched by an electron transfer process, while the NADH-modified QDs are inefficiently quenched by the reduced cofactor. These properties enable the implementation of the QDs for the fluorescence analysis of ethanol in the presence of alcohol dehydrogenase. The NADH-functionalized QDs were used for the optical analysis of the 1,3,5-trinftrotriazine, RDX explosive, with a detection limit that corresponded to 1 × 10-10 M. We demonstrate cooperative optical and catalytic functions of the core-shell components of the QDs in the analysis of RDX.

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NAD/NADH-Sensitive quantum dots: Applications to probe nad-dependent enzymes and to sense the rdx explosive

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