Abstract
A three-dimensional composite consisting of the oxygen-insensitive flavin-dependent glucose dehydrogenase, GDH, and Au nanoparticles (NPs) is assembled on a Au surface using an electropolymerization process. The bis-aniline-cross-linked GDH/Au NPs composite reveals effective electrical contact with the electrode (ket = 1100 s-1), and the effective bioelectrocatalyzed oxidation is driven by the enzyme/NPs matrix. The GDH/Au NPs-functionalized electrode is implemented as an amperometric glucose sensor, and it reveals superior functions when compared to an analogous glucose oxidase/Au NPs system. The O2-insensitive GDH/Au NPs composite electrode was further used as an anode in a membraneless glucose/O2 biofuel cell. The cathode in this system was composed of bilirubin oxidase cross-linked onto a carbon nanotube-modified glassy carbon electrode. The power output of the cell was 32 μW cm-2.
Original language | English |
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Pages (from-to) | 2385-2391 |
Number of pages | 7 |
Journal | ACS Nano |
Volume | 5 |
Issue number | 3 |
DOIs | |
State | Published - 22 Mar 2011 |
Keywords
- Au nanoparticles
- biofuel cell
- biosensor
- electropolymerization
- glucose
- glucose dehydrogenase