Nano-engineered flavin-dependent glucose dehydrogenase/gold nanoparticle-modified electrodes for glucose sensing and biofuel cell applications

Omer Yehezkeli, Ran Tel-Vered, Sara Raichlin, Itamar Willner*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

203 Scopus citations

Abstract

A three-dimensional composite consisting of the oxygen-insensitive flavin-dependent glucose dehydrogenase, GDH, and Au nanoparticles (NPs) is assembled on a Au surface using an electropolymerization process. The bis-aniline-cross-linked GDH/Au NPs composite reveals effective electrical contact with the electrode (ket = 1100 s-1), and the effective bioelectrocatalyzed oxidation is driven by the enzyme/NPs matrix. The GDH/Au NPs-functionalized electrode is implemented as an amperometric glucose sensor, and it reveals superior functions when compared to an analogous glucose oxidase/Au NPs system. The O2-insensitive GDH/Au NPs composite electrode was further used as an anode in a membraneless glucose/O2 biofuel cell. The cathode in this system was composed of bilirubin oxidase cross-linked onto a carbon nanotube-modified glassy carbon electrode. The power output of the cell was 32 μW cm-2.

Original languageEnglish
Pages (from-to)2385-2391
Number of pages7
JournalACS Nano
Volume5
Issue number3
DOIs
StatePublished - 22 Mar 2011

Keywords

  • Au nanoparticles
  • biofuel cell
  • biosensor
  • electropolymerization
  • glucose
  • glucose dehydrogenase

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