Nanobiotechnology is a multidisciplinary field that covers a vast and diverse array of technologies coming from engineering, physics, chemistry, and biology. It is the combination of these fields that has led to the birth of a new generation of materials and methods of making them. The scope of applications is enormous and every day we discover new areas of our daily lives where they can find use. This chapter aims to provide the reader with a brief overview of nanobiotechnology by describing different aspects and approaches in research and application of this exciting field. It also provides a short list of recently published review articles and books on the different topics in nanobiotechnology.