Nanoscale Plasmonic V-Groove Waveguides for the Interrogation of Single Fluorescent Bacterial Cells

Oren Lotan, Jonathan Bar-David, Cameron L.C. Smith, Sharon Yagur-Kroll, Shimshon Belkin, Anders Kristensen, Uriel Levy*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

9 Scopus citations


We experimentally demonstrate the interrogation of an individual Escherichia coli cell using a nanoscale plasmonic V-groove waveguide. Several different configurations were studied. The first involved the excitation of the cell in a liquid environment because it flows on top of the waveguide nanocoupler, while the obtained fluorescence is coupled into the waveguide and collected at the other nanocoupler. The other two configurations involved the positioning of the bacterium within the nanoscale waveguide and its excitation in a dry environment either directly from the top or through waveguide modes. This is achieved by taking advantage of the waveguide properties not only for light guiding but also as a mechanical tool for trapping the bacteria within the V-grooves. The obtained results are supported by a set of numerical simulations, shedding more light on the mechanism of excitation. This demonstration paves the way for the construction of an efficient bioplasmonic chip for diverse cell-based sensing applications.

Original languageAmerican English
Pages (from-to)5481-5488
Number of pages8
JournalNano Letters
Issue number9
StatePublished - 13 Sep 2017

Bibliographical note

Publisher Copyright:
© 2017 American Chemical Society.


  • Aluminum plasmonics
  • bacterial biosensors
  • bio-plasmonic chips
  • fluorescent bacterial cells
  • nanoscale plasmonic V-groove waveguides
  • single-bacterium excitation


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