TY - JOUR
T1 - Native Mass Spectrometry of Recombinant Proteins from Crude Cell Lysates
AU - Gan, Jinrui
AU - Ben-Nissan, Gili
AU - Arkind, Galina
AU - Tarnavsky, Mark
AU - Trudeau, Devin
AU - Noda Garcia, Lianet
AU - Tawfik, Dan S.
AU - Sharon, Michal
N1 - Publisher Copyright:
© 2017 American Chemical Society.
PY - 2017/4/18
Y1 - 2017/4/18
N2 - Determining the properties of proteins prior to purification saves time and labor. Here, we demonstrate a native mass spectrometry approach for rapid characterization of overexpressed proteins directly in crude cell lysates. The method provides immediate information on the identity, solubility, oligomeric state, overall structure, and stability, as well as ligand binding, without the need for purification.
AB - Determining the properties of proteins prior to purification saves time and labor. Here, we demonstrate a native mass spectrometry approach for rapid characterization of overexpressed proteins directly in crude cell lysates. The method provides immediate information on the identity, solubility, oligomeric state, overall structure, and stability, as well as ligand binding, without the need for purification.
UR - http://www.scopus.com/inward/record.url?scp=85020017312&partnerID=8YFLogxK
U2 - 10.1021/acs.analchem.7b00398
DO - 10.1021/acs.analchem.7b00398
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C2 - 28345863
AN - SCOPUS:85020017312
SN - 0003-2700
VL - 89
SP - 4398
EP - 4404
JO - Analytical Chemistry
JF - Analytical Chemistry
IS - 8
ER -