Native Mass Spectrometry of Recombinant Proteins from Crude Cell Lysates

Jinrui Gan, Gili Ben-Nissan, Galina Arkind, Mark Tarnavsky, Devin Trudeau, Lianet Noda Garcia, Dan S. Tawfik, Michal Sharon*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

43 Scopus citations


Determining the properties of proteins prior to purification saves time and labor. Here, we demonstrate a native mass spectrometry approach for rapid characterization of overexpressed proteins directly in crude cell lysates. The method provides immediate information on the identity, solubility, oligomeric state, overall structure, and stability, as well as ligand binding, without the need for purification.

Original languageAmerican English
Pages (from-to)4398-4404
Number of pages7
JournalAnalytical Chemistry
Issue number8
StatePublished - 18 Apr 2017
Externally publishedYes

Bibliographical note

Publisher Copyright:
© 2017 American Chemical Society.


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