Native Mass Spectrometry of Recombinant Proteins from Crude Cell Lysates

Jinrui Gan; Gili Ben-Nissan; Galina Arkind; Mark Tarnavsky; Devin Trudeau; Lianet Noda Garcia; Dan S. Tawfik; Michal Sharon

doi:10.1021/acs.analchem.7b00398

Native Mass Spectrometry of Recombinant Proteins from Crude Cell Lysates

Jinrui Gan
, Gili Ben-Nissan
, Galina Arkind
, Mark Tarnavsky
, Devin Trudeau
, Lianet Noda Garcia
, Dan S. Tawfik
, Michal Sharon^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

52 Scopus citations

Abstract

Determining the properties of proteins prior to purification saves time and labor. Here, we demonstrate a native mass spectrometry approach for rapid characterization of overexpressed proteins directly in crude cell lysates. The method provides immediate information on the identity, solubility, oligomeric state, overall structure, and stability, as well as ligand binding, without the need for purification.

Original language	English
Pages (from-to)	4398-4404
Number of pages	7
Journal	Analytical Chemistry
Volume	89
Issue number	8
DOIs	https://doi.org/10.1021/acs.analchem.7b00398
State	Published - 18 Apr 2017
Externally published	Yes

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© 2017 American Chemical Society.

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10.1021/acs.analchem.7b00398

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abstract = "Determining the properties of proteins prior to purification saves time and labor. Here, we demonstrate a native mass spectrometry approach for rapid characterization of overexpressed proteins directly in crude cell lysates. The method provides immediate information on the identity, solubility, oligomeric state, overall structure, and stability, as well as ligand binding, without the need for purification.",

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AU - Ben-Nissan, Gili

AU - Arkind, Galina

AU - Tarnavsky, Mark

AU - Trudeau, Devin

AU - Noda Garcia, Lianet

AU - Tawfik, Dan S.

AU - Sharon, Michal

PY - 2017/4/18

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AB - Determining the properties of proteins prior to purification saves time and labor. Here, we demonstrate a native mass spectrometry approach for rapid characterization of overexpressed proteins directly in crude cell lysates. The method provides immediate information on the identity, solubility, oligomeric state, overall structure, and stability, as well as ligand binding, without the need for purification.

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Native Mass Spectrometry of Recombinant Proteins from Crude Cell Lysates

Abstract

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