Native RNA Immunoprecipitation (RIP) for Precise Detection and Quantification of Protein-Interacting RNA

Mai Baker, Rami Khosravi, Maayan Salton*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

Abstract

Proteins with either RNA or DNA-binding motifs were shown to bind RNA. Immunoprecipitation of such proteins using antibodies and identification of the RNA-binding molecules is called RNA immunoprecipitation (RIP). The RNA precipitated with the studied protein can be detected by real-time polymerase chain reaction (PCR), microarray or sequencing. Here, we detail a method for native immunoprecipitation, without cross-linking, to isolate protein-RNA complexes followed by subsequent extraction and quantification of the co-purified RNA.

Original languageAmerican English
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages107-114
Number of pages8
DOIs
StatePublished - 2023

Publication series

NameMethods in Molecular Biology
Volume2666
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Bibliographical note

Publisher Copyright:
© 2023, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Keywords

  • Antibody
  • Native RNA immunoprecipitation
  • RNA extraction
  • RNA-binding protein
  • Real-time PCR

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